|Budget Amount *help
¥3,300,000 (Direct Cost : ¥3,300,000)
Fiscal Year 2000 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1999 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 1998 : ¥2,300,000 (Direct Cost : ¥2,300,000)
In order to carry out the title project, we collected a large number of specimens of soft corals of the genus Sarcophyton and some sponges of the genera such as Stylotella and Dysidea in the coral reefs along the Ryukyu Islands. Each specimen was extracted and checked with gradient HPLC and ^1H NMR for major constituents. We focused on the cembranoid diterepenes in the soft corals with respect to species, seasons, and geographic regions. As a result, the most abundant species S.glaucum was found to be three dominant chemotypes containing emblide, 2R,7S,8S-sarcophytoxide, and 7-hydroxy-1,3,11-cembratrien-20,8- carbolactone together with several rare chemotypes. In case of S.trocheliophorum, the major chemotype was 2R,7R,8R-sarcophytoxide, while S.ehrenbergi was found to be 7,8-epoxy- 1,3,11-cembratriene. Some other species, S.crassocaule, S.elegans, S.infundibuliforme, S.pulchellum, S.cherbonnieri, were also investigated. Seasonal change was not observed for most species, however, geographic variation may exist in some species.