KODAMA Keiichi SHOOL OF MEDICINE KEIO UNIVERSITY, DEPARTMENT OF INTERNAL MEDICINE, POST GRADUATE, 医学部・内科, 助手 (70276236)
MIYAMOTO Kazunori SHOOL OF MEDICINE KEIO UNIVERSITY, DEPARTMENT OF INTERNAL MEDICINE, POST GRADUATE, 医学部・内科, 助手 (70265784)
MURATA Mitsuru SHOOL OF MEDICINE KEIO UNIVERSITY, DEPARTMENT OF INTERNAL MEDICINE, ASSOCIATE PROFESSOR, 医学部・内科, 講師 (50174305)
大橋 徳巳 慶應義塾大学, 医学部, 助手 (50255444)
|Budget Amount *help
¥1,800,000 (Direct Cost : ¥1,800,000)
Fiscal Year 1999 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1998 : ¥1,200,000 (Direct Cost : ¥1,200,000)
(Purpuse) : Diabetes mellitus is related to vessel damage which are contained both microangiopathy and macroangiopathy. However, this mechanism has been unknoun, so, we examined genetic analysis of them for clarified mechanism.
(Method) : 290 diabetic patients were selected by the criteria of WHO 1985 about diabetes and were obtained informed consent. Retinopathy was diagnosed by ophthalmologist, diabetic nephropathy was decided due to the decision of national health department in Japan. Cardiovascular disease was decided by tredmill test and past history, and cerebral artery disease was dianosed by brain MRI examinations and past history. Genetic polymorphism was measured by direct polymerase chain amplification about clolesterol ester tranfer protein (CETP) Tag IB, parooxonase 192AKG/gln, platelet membrane GPIb/Iy 145T/1T, LDL : Pvu-II Hin III ser/step, plasminogen activator inhibitor (PAI-1), 5HT2A receptor, methylene tetrahydroforate reductase (MTHFR), e-NOS, ApoE, fibrinogen, mitodondrial 5178 and ACE Insertion/Deletion.
(Results and Conclusion) : Results of 12 genetic analysises, CETP was significantly related to macroangiopathy and serum LDL concentration. Paraoxonase Arg/Arg was significant high risk in diabetics with macroangiopathy. PAI-1 4G/4G was related to hypertension, but not correletion with micro and macroangiopathy. 5HT2A c/c, c/T was significant high risk at macroangiopathy by calcification degree using aorta CT scan (TES-100method). MTHFR T/T was related to macroangiopathy. On conclusion, several genetics were related to diabetic angiopathy.