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Real time imaging of neuronal excitation and cellular morphology of plastic synapses

Research Project

Project/Area Number 10680741
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionFukui University

Principal Investigator

MURASE Kazuyuki  Human and Artificial Intelligent Systems, Fukui University, Professor, 工学部, 教授 (40174289)

Co-Investigator(Kenkyū-buntansha) ASAI Tatsuya  Human and Artificial Intelligent Systems, Fukui University, Lecturer, 工学部, 講師 (60291374)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsSynaptic Plasticity / Long-term Potentiation / Pain / Spinal Cord / Con-focal Microscope / Optical Recording / Slice Preparation / Sensation / レーザースキャン共焦点顕微鏡 / 樹状突起 / 蛍光染色 / NMDA / オピオイド
Research Abstract

It is now believed that the long-term depression of synaptic transmissions from primary afferent C fibers to spinal dorsal horn neurons after conditioning low-frequency stimulation of A fibers take a critical role in the analgesia by adequate electrical or tactile stimulation. It is also known that dendritic morphology of some dorsal horn neurons is altered after the application of substance P, the pain transmitter/modulator released from pain-conducting primary afferent fibers. Therefore, it has been speculated that the morphological change might be caused by hyperalgesia and/or allodynia, and that it might be reset by analgesics such as morphine. The purpose of the present research is thus to reveal the properties of neuronal plasticity in the spinal dorsal horn. Especially, plastic changes in the neuronal excitation and neuronal morphology in the substantia gelatinosa of the dorsal horn are to be studied by using optical imaging methods with voltage sensitive dyes and con-focal micr … More oscope.
We have revealed that conditioning high-frequency stimulation of A fibers in the dorsal root robustly changed C-fiber-evoked neuronal excitation, a facilitation under a low activity of inhibitory interneurons containing inhibitory amino acids, and suppression at the high activity. Conditioning low-frequency stimulation inhibited the induction and maintenance of the high-frequency conditioning-induced facilitation. Dendritic varicosities of some dorsal horn neurons in the gelatinosa, stained with DiI by the micro oil-drop method and observed with a laser-scan con-focal microscope, were expanded after the application of conditioning.
These results thus exhibited clearly by optical imaging techniques, plastic changes in the dorsal horn, both in the signal transmission and in the neuronal morphology, take place by the conditioning stimulations known to induce hyperalgesia, allodynia or analgesia, and strongly indicated that such plasticity might be essential for the induction and transmission of the pain. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] H. Ikeda, K. Murase: "Robust changes of afferent-induced excitation in the rat spinal dorsal horn after conditioning high-frequency stimulation"Journal of Neurophysiology. 83. 2412-2420 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Ikeda, T. Asai, M. Randic, K. Murase: "Robust suppression of afferent-induced excitation in the rat spinal dorsal horn after conditioning low-frequency stimulation"Journal of Neurophysiology. 82. 1957-1964 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Murase, T. Saka, T. Asai, H. Ikeda: "Functional circuitry for the induction of prolonged excitation in the rat spinal dorsal horn"European Journal of Neuroscience. 11. 3355-3358 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Kita, K. Oda, K. Murase: "Effects of dopamine agonists and antagonists on optical responses recorded in rat frontal cortex slices after stimulation of the subcortical white matter"Experimental Brain Research. 14. 61-66 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Ikeda, T. Asai, K. Murase: "Plasticity of the C fiber-induced excitation in the rat spinal dorsal horn in slice"Pain Research. 14. 61-66 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Murase, T. Saka, S. Terao, H. Ikeda, T. Asai: "Slow intrinsic optical signals in rat spinal dorsal horn in slice"NeuroReport. 9. 3663-3667 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Ikeda, PD. Ryu, JB. Park, M. Tanifuji, T. Asai, K. Murase: "Optical responses evoked by single-pulse stimulation to the dorsal root in the rat spinal dorsal horn in slice"Brain Research. 812. 81-90 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Ikeda, K. Murase: "Robust changes of afferent-induced excitation in the rat spinal dorsal horn after conditioning high-frequency stimulation"Journal of Neurophysiology. 83. 2412-2420 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Ikeda, T. Asai, M. Randic, K. Murase: "Robust suppression of afferent-induced excitation in the rat spinal dorsal horn after conditioning low-frequency stimulation"Journal of Neurophysiology. 82. 1957-1964 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Murase, T. Saka, T. Asai, H. Ikeda: "Functional circuitry for the induction of prolonged excitation in the rat spinal dorsal horn"European Journal of Neuroscience. 11. 3355-3358 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Kita, K. Oda, K. Murase: "Plasticity of the C fiber-induced excitation in the rat spinal dorsal horn in slice"Experimental Brain research. 125. 383-388 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Ikeda, T. Asai, K. Murase: "Plasticity of the C fiber-induced excitation in the rat spinal dorsal horn in slice"Pain Research. 14. 61-66 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Murase, T. Saka, S. Terao, H. Ikeda, T. Asai: "Slow intrinsic optical signals in rat spinal dorsal horn in slice"NeuroReport. 9. 3663-3667 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Ikeda, PD. Ryu, JB. Park, M. Tanifuji, T. Asai, K. Murase: "Optical responses evoked by single-pulse stimulation to the dorsal root in the rat spinal dorsal horn in slice"Brain Research. 812. 81-89 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Ikeda,K.Murase: "Robust changes of afferent-induced excitation in the rat spinal dorsal horn after conditioning high-frequency stimulation"Journal of Neurophysiology. 83(4). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Ikeda,T.Asai,M.Randic,K.Murase: "Robust suppression of afferent-induced excitation in the rat spinal dorsal horn after conditioning low-frequency stimulation"Journal of Neurophysiology. 82. 1957-1964 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Murase,T.Saka,T.Asai,H.Ikeda: "Functional circuitry for the induction of prolonged exicitation in the rat spinal dorsal horn"European Journal of Neuroscience. 11. 3355-3358 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Kita,K.Oda,K.Murase: "Effects of dopamine agonists and antagonists on optical responses recorded in rat frontal cortex slices after stimulation of the subcortical white matter"Experimental Brain Research. 125. 383-388 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Ikeda,T.Asai,K.Murase: "Plasticity of the C fiber-induced excitation in the rat spinal dorsal horn in slice"Pain Research. 14. 61-66 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Murase,T.Saka,S.Terao,H.Ikeda,T.Asai: "Slow intrinsic optical signals in rat spinal dorsal horn in slice"NeuroReport. 9. 3663-3667 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Ikeda,PD.Ryu,JB.Park,M.Tanifuji,T.Asai,K.Murase: "Optical responses evoked single-pulse stimulation to the dorsal root in the rat spinal dorsal horn in slice"Brain Research 812. 812. 81-90 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murase,Kazuyuki: "Robust restructuring of dendrites induced by N-methyl-D-aspartate in rat spinal dorsal horn observed in real time" Japanese Society for Neuroscience Abstract Neuroscience Research. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Murase,Kazuyuki: "Slow intrinsic optical signals in rat spinal dorsal horn in slice" NeuroReport. 9. 3663-3667 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ikeda,Hiroshi: "Optical responses evoked by single-pulse stimulation to the dorsal root in rat spinal dorsal horn in slice" Brain Research. 812. 81-90 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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