| Project/Area Number |
11306004
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | Nagoya University |
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Principal Investigator |
DOKE Noriyuki Nagoya University, Graduate School of Bioagricultural Sciences Professor, 大学院・生命農学研究科, 教授 (80023472)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Hirofumi Nagoya University, Graduate School of Bioagricultural Sciences Assistant Professor, 大学院・生命農学研究科, 助手 (30240245)
KAWAKITA Kazuhiko Nagoya University, Graduate School of Bioagricultural Sciences Associate Professor, 大学院・生命農学研究科, 助教授 (90186065)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥42,310,000 (Direct Cost: ¥40,300,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2001: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2000: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1999: ¥27,500,000 (Direct Cost: ¥27,500,000)
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| Keywords | Oxidative burst / NADPH oxidase gene / gp91phox homologue / Systemic signal transduction / Gene-silencing / Cloning of defense genes / systemic induced resistance / Gulucose-6-phosphate dehydrogenase / 防御応答 / NADPH酸化酵素 / グルコース-6-リン酸脱水素酵素 / カルシウムインフラクス / 活性酸素生成NADPH酸化酵素 / gp91^<phox>ホモログのクローニング / HMGRプロモーター / セスキテルペノイドシクラーゼ / 硝酸還元酵素遺伝子 / 防御応答遺伝子の発現 / NADPH酸化酵素活性化機構 / 細胞の過敏感反応能 / 防御応答遺伝子のクローニング / Ca^<2+>シグナリング |
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| Research Abstract |
Molecular basis of oxidative burst (OXB) system, which was an initial signal for induction of defense response in plants, regulatory mechanisms for activation of the system, and dynamic phases of molecular and physiological changes associated with local or systemic induced resistance were investigated for understanding of a systematic mechanism of plant active defense under the control by oxidative burst Following results were obtained :
1 ) Two gp91phox horologes, which may be a subunit of NADPH oxidase, were cloned from potato and tobacco plants, respectively, and they were characterized by membrane fern proteins with binding sites of NADPH and calcium,
2 ) The OXB system was rapidly activated from inactive state by wounding or infection stimuli and OXB in the potentiated cells was inhibited by Ca^<2+> channel blockers, calmodulin inhibitors or protein kinase inhibitors, suggesting involvement of a C kinase in induction of OXB.
3 ) Defense-metabolism-related genes, which were stimulated by OXB-inducible elicitors, were cloned by mean of differential display or subtraction,
4 ) Gene silencing about some of the cloned genes by using a PVX vector demonstrated that genes responsible for OXB or NADPH producer were a crucial role in defense expression against incompatible Phytophthora fungus.
5 ) Induction of local OXB caused local as well as systemic induced resistance, dispatching a noble systemic signal which caused a sequential Ca^<2+> influx from cells to cells in non potentiated tissues and then induced systemic OXB at the potentiated tissue for triggering induction of systemic acquired resistance.
6 ) A positive regulation of induction of OXB system by signal dispatching elements was suggested to be applicable for induction of plant immunization against pathogens.
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