| Project/Area Number |
11306021
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
FUJITA Shoichi Hokkaido Univ. Grad. School of Vet. Sci., 大学院・獣医学研究科, 教授 (10143314)
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| Co-Investigator(Kenkyū-buntansha) |
HIDENOBU Hoshi Osaka Prefecture Univ. College of Agriculture, 農学部, 助手 (30301188)
ISHIZUKA Mayumi Hokkaido Univ. Grad. School of Vet. Sci., 大学院・獣医学研究科, 助手 (50332474)
KAZUSAKA Akio Hokkaido Univ. Grad. School of Vet. Sci., 大学院・獣医学研究科, 助教授 (00002113)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥41,770,000 (Direct Cost: ¥39,100,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2002: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2001: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2000: ¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 1999: ¥20,900,000 (Direct Cost: ¥20,900,000)
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| Keywords | Chlorinated hydrocarbon / tri-butyltin / fish-eating / Cytochrome P450 / wild life / Steroid hormone / organochlorine / endocrine disrupting chemicals / 環境汚染 / ダイオキシン受容体 / PPAR / エストロゲン / ベンゾピレン / ビスフェノールA / CYP1A1 / ゴマフアザラシ / クラカケアザラシ / オオワシ / オジロワシ / P450 / コイ / 二次卵母細胞 / 異物代謝酵素CYP / バイオマーカ / CYP2B / アロマターゼ活性 |
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| Research Abstract |
In this study, we investigated the accumulation levels of environmental pollutants and their effects on the hormonal homeostasis in reproductive organs and livers of wild life or experimental animals. Especially, we focused on the effects of environmental pollutants on fish-eating wild life, since numerous lipophilic compounds were accumulated in these species.
Chlorinated hydrocarbon (CHCs) levels in the blubber of Larga seals and Ribbon seals collected from the coastal waters of Hokkaido, Japan were determined in order to assess the hormonal effects of CHC exposure. In Ribbon seals, total T3 levels significantly decreased with an increase of di-ortho PCB (PCB170 and 180) residues. We also determined the organochlorine concentrations in the breaset muscle of Steller's sea eagles and white-tail sea eagles. We detected PCB, DDT and other organopesticides at high levels, which was possible to cause reproductive toxicity. Furthermore, we investigated the accumulation levels of other heavy metals and detected tri-butyltin (TBT) in wild life at high concentration. Thus, we investigated the effects of TBT on the developing experimental animals. The pregnant rats were administrated corn oil or TBT from gestation day (GD 5) through postnatal day (PND) 25. The glial cell differentiations in hypothalamus and cerebellum of pups were transiently suppressed by TBT treatment.
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