| Project/Area Number |
11480224
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
WATANABE Masahiko Hokkaido Univ., School of Medicine, Prof., 大学院・医学研究科, 教授 (70210945)
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| Co-Investigator(Kenkyū-buntansha) |
NAGASHIMA Masabumi Hokkaido Univ., School of Medicine, Asso. Prof., 大学院・医学研究科, 助教授 (40241319)
INOUE Kaoru Hokkaido Univ., College of Medical Technology, Prof., 医療技術短期大学部, 教授 (80133718)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2000: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥9,200,000 (Direct Cost: ¥9,200,000)
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| Keywords | glutamate receptor / mGLuR1 / GluRδ2 / cerebellum / Purkinje cell / synapse formation / development / knockout mouse / PLCβ4 / マウス |
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| Research Abstract |
In the research project, we studied metabotropic glutamate signaling to reveal the molecular anatomy and its role in synapse development and plasticity. We analyzed the following items; 1) GTP-binding protein and phospholipases that function at Purkinje cell synpases, 2) Purkinje cell synapse formation in knockout mice lacking GTP-binding protein and phospholipase, 3) the form of persistent multiple innervation in miceiacking mGluR1 and GluRδ2, and 4) NMDA-type glutamate receptors at granule cell synapses. Distinct synapse localization of these signaling molecules were clarified. Moreover, we found distinct roles played by mGluR1 and GluRδ2 in the elimination process of surplus climbing fibers. mGluR1 signaling is essential to homotypic competition among climbing fibers for the proximal dendritic segment of Purkinje cells, whereas GluRδ2 is important in heterotypic competition between parallel fibers and climbing fibers. With both functions, Purkinje cells can establish the territorized innervation underlying LTD induction and cerebellar motor learning.
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