| Project/Area Number |
11556016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Kobe University |
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Principal Investigator |
OHKAWA Hideo Research Center for Environmental Genomics, Professor, 遺伝子実験センター, 教授 (90233048)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAKE Shiro Horiba Biotechnology Inc., Research Manager, 開発・営業部長(研究職)
KAWATA Mitsuyasu Otsuka Chemical Co., Ltd., Naruto Research Center, Research Scientist, 鳴門研究所, 主任研究員
KATAGIRI Masanao Osaka Kyoiku University, Division of Natural Science, Associate Professor, 教育学部, 助教授 (00185802)
中田 昌伸 神戸大学, 農学部, 教授 (40031190)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2000: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | ELISA / monoclonal antibody / scFv / endocrine disruptor / simazine / bisphenol A / alachlor / nonylphenol / 外因性内分泌攪乱物質 / 外因性内分泌撹乱物質 / シメトリン / アラクロール / パラニトロフェノール / 可変領域cDNA / 有機リン殺虫剤 |
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| Research Abstract |
Contamination of the environment and agricultural products with environmental endocrine disruptors is a serious global problem. These chemicals were found in the environment and agricultural products at nano-level concentrations and suspected of affecting ecosystems and human health. Therefore, it is requested to develop novel technologies to monitor contaminations by nano-levels of environmental endocrine disruptors since most conventional technologies are high cost, labor intensive and taking time to obtain results, and do not work effectively to monitor nano-level contaminants.
(1) We prepared monoclonal antibodies specific to the endocrine disruptors bisphenol A, and nonylphenol, and the herbicides alachlor and simazine, and then developed enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies. The ELISA methods developed were highly sensitive, convenient and low-cost as compared with the conventional analytical methods.
(2) We also developed recombinant scFv antibodies based on the monoclonal antibodies by genetic engineering and used for preparation of affinity columns for clear-up of the extracts of samples of soil and agricultural products.
(3) It was found that the combined use the novel ELISA method with the affinity column produced highly efficient monitoring data for environmental endocrine disruptors in a various samples.
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