| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
|
|---|
| Research Abstract |
Genetic expression of mRNA, which indicates generation of the secretion for self-healing and protection against the injury, is detected in a plant inflicted by insects. The same mRNA is detected in the other plant, which is not damaged by the insects and is just around the injure plant. This suggests that communication mechanism or route exists for transmitting the injury crisis signal to other plants.
To reveal the inter-plant communication mechanism or routes, we have developed several measuring and analysis systems, that is, genetic analysis system, bio-photon imaging and counting system, chlorophyll fluorescence measuring system , bio-electric potential signal acoustic emission signal measuring system, photo-sythesis/transpiration measuring system and a shielded cabin with air-tight partition shutter, dividing the cabin into two rooms , each for the plant with injury and without injury repectively.
When pin-point hot (50℃) or cold (5℃) stimuli is given on a leaf of Kapok (Schefflera arboricola). bio-electric potential signal, chlorophyll fluorescence and photosynthetic response on the same leaf is affected remarkably. When the same pin-point hot (50℃) or cold (5℃) stimuli is given on one of the leaves, quite similar responses mentioned above are observed on the other leaves at 20℃ without stimuli.
This suggests that hot or cold stimuli given on certain leaf is transmitted to the other leave. Siginificant difference has been confirmed in the data between those of mRNA detected for the leaves of rice plant injured by insects and those without injury. The same difference has been observed between the data for bio-photon on the leaves with and without injury.
Availablity and effectiveness of the method of integrating the genetic analysis scheme and other non-destructive, real time opto-elctronic measuring skills has been proved for the study of the inter-plant communication mechanisms or routes.
|
