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Affect of chronic and acute stress on growth and survival of hatchery-reared Japanese flounder juveniles

Research Project

Project/Area Number 11660195
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionFUKUI PREFECTURAL UNIVERSITY

Principal Investigator

TOMINAGA Osamu  MARINE BIOSCIENCE, FUKUI PREFECTURAL UNIVERSITY, Associate Professor, 生物資源学部, 助教授 (90264689)

Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsHatchery-reared Japanese flounder / chronic stress / Acute stress / stress response / cortisol / ACTH cell / Interrenal cell / lethal stressor / 輸送ストレス / メラニン量 / ACTH免疫染色 / マクロファージ / 致死限界ストレッサー / 血漿中グルコース / ヘマトクリット値 / Na^+ / K^+ATPase活性 / ストレッサー / 白血球 / ACTH
Research Abstract

1. Physiological and morphological response for chronic stress
Post-settlement juveniles were reared for 3 months at three different rearing density. Plasma cortisol level of young fish was not different at the end of the experiment among three treatments but the nuclear area of interrenal cell of fish reared under overcrowded conditions became significantly larger. It suggested that chronic stress promoted metabolism of cortisol. To examine a change in capability of ACTH secretion for chronic stress, juveniles were reared for 12 weeks under overcrowded conditions without sand on the bottom. In this experiment, the nuclear area of ACTH secretion cell significantly increased in size. Thus, morphological change of juveniles was recognized for chronic stress at cell level.
2. Response of juveniles for lethal acute-stressors
To determine a tolerance to acute stress, flounder juveniles were exposed to the air. A mortality of juveniles which were exposed to the air for 15 and 20 minutes was about 65 % and 30 %, respectively. All individuals were dead by 30 minutes exposure to the air. All juveniles which were exposed to the air for 10 minutes once a day for five days before 15 minutes exposure were survived. However, a survival rate of fish which were not stressed before exposure was only 13. 3 %. As a cortisol elevation after exposure was not observed in survival juveniles, it was suggested that tolerance to acute stress was related to the cortisol response.
3. Effect of handling at transportation on mortality
Small size juveniles (35 mm TL) showed clear stress response (cortisol elevation ) for handling at transportation from grow-out facility to release area and mortality rate was high. It was suggested that transportation is one factor of mortality after release for small size fish.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 富永修: "人工種苗の放流による健苗性の評価"平成10年度健苗育成技術開発研究成果の概要. 102-113 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 富永修: "人工種苗の放流による健苗性の評価"平成11年度健苗育成技術開発研究成果の概要. 99-112 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tominaga, Osamu: "Evaluation of seedling quality of hatchery-reared Japanese flounder juveniles judging grome a mass release experiment"Heisei 10 nendo Kenbyo Ikusei Gijutu Kaihatsu Kenkyu Seika no Gaiyu (Progress report on technology of healthy seedling production). 102-113 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tominaga, Osamu: "Evaluation of seedling quality of hatchery-reared Japanese flounder juveniles judging grome a mass release experiment"Heisei 11 nendo Kenbyo Ikusei Gijutu Kaihatsu Kenkyu Seika no Gaiyu (Progress report on technology of healthy seedling production). 99-112 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary

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Published: 1999-04-01   Modified: 2016-04-21  

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