Diversity of cholinergic neurons in the human central nervous system
| Project/Area Number |
11670171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kanazawa University |
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Principal Investigator |
YOSHIO Oda Faculty of Medicine Kanazawa University Associate Professor, 医学部・医学科, 助教授 (70169316)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Vesicular acetylcholine transporter / Choline acetyltransferase / Immunohistochemistry / In situ hybridization / RT-PCR / Human / Central nervous system / Human brain |
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| Research Abstract |
Choline acetyltransferase (ChAT), an enzyme for biosynthesis of acetylcholine, and vesicular acetylcholine transporter (VAChT), a molecule for the translocation of cytoplasmic acetylcholine into synaptic vesicles, are known to be good markers for cholinergic neurons. In order to investigate whether cholinergic neurons are diverse with relation to localization and function, immunohistochemical and in situ hybridization studies were performed on the human central nervous by using specific antibodies and RNA probes for human ChAT and VAChT.In this study, an antibody to human recombinant VAChT protein was developed, which was applicable to paraffin-embedded tissue sections. The distributions of ChAT mRNA, ChAT protein, VAChT mRNA and VAChT protein were similar, but not exactly the same in the human central nervous system. In addition, cholinergic projection neurons tended to express these molecules more than cholinergic local circuit neurons, which might be related to the different functional levels between the two types of cholinergic neurons. Southern blot analysis following reverse transcription- polymerase chain reaction revealed that the caudate nucleus contained two R-type mRNAs, but not N-or M-type mRNA.Our previous study demonstrated that in the human spinal cord M-type mRNA is much more abundant than R-type mRNA.These results suggest that transcription mechanisms of ChAT are different in cholinergic neurons between the caudate nucleus and spinal cord.
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Report
(3 results)
Research Products
(10 results)
