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AN ATTEMPT TO INTRODUCE GLYCOBIOLOGICAL OR BIOCHEMICAL TECHNIQUES INTO FORENSICPATHOLOGICAL DIAGNOSIS

Research Project

Project/Area Number 11670413
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionSHIGA UNIVERSITY OF MEDICAL SCIENCE

Principal Investigator

NISHI Katsuji  SHIGA UNIVERSITY OF MEDICAL SCIENCE, FACULTY OF MED,PROF., 医学部, 教授 (60073681)

Co-Investigator(Kenkyū-buntansha) USHIYAMA Ikuko  SHIGA UNIVERSITY OF MEDICAL SCIENCE, FACULTY OF MED,ASSISTANT, 医学部, 助手 (70283562)
YAMOMOTO Yoshio  SHIGA UNIVERSITY OF MEDICAL SCIENCE, FACULTY OF MED,LECTURE, 医学部, 助手 (60111902)
NISHIMURA Akiyoshi  SHIGA UNIVERSITY OF MEDICAL SCIENCE, FACULTY OF MED,ASS.PROF, 医学部, 助教授 (60283561)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsglycoconjugate / carbohydrate chain / degenerative deposition / histochemistry / brain disorder / レクチン / ドーパミン / ペプチダーゼ / 糖鎖構造物質
Research Abstract

Lectin histochemical technique clearly revealed the existence of senile plaque, neurofibrillary tangles, corpora amylacea, and three types of carbohydrate depositions, that is, spherical, vascular and stratiform type, in the human brain from patients with brain disorder. The spherical type deposits were detected in the molecular layer of the dentate gyrus of the hippocampal formation of patients with schizophrenia. The vascular and stratiform type which showed amorphous in the hematoxylin eosin staining were detected in the white matter region in the cerebrum and cerebellum of patients with brain disorder, such as dementia, Down's syndrome or brain edema. Dipeptidyl peptidase III (DPIII) and alanyl aminopeptidase (AP) were purified from human liver cytosol, and antibodies against these enzymes were produced. Antibody against AP histochemically reacted with neurons and glial cells, and could stain the above mentioned amorphous depoditions. The depositions newly detected in this project may be results from the disturbance of glycogenesis or blood brain barrier in the brain and relate to brain disorder. Antibodies against serotonin 2A receptor and tyrosine hydroxylase revealed that many neurons in the human ventral tegment area and substantia nigra are doubly labeled and suggested that activity of human midbrain dopaminergic neurons might be strongly regulated via serotonin 2A receptors at the level of their originating nuclei.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Ohkubo I.,Li Y-H.,Maeda T.,Yamamoto Y.,Yamane T.,Nishi K.,: "Dipeptidyl peptidase III from rat liver cytosol : Purification, molecular cloningand immunohistochemical localization."Biol.Chem.. 380. 1421-1430 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikemoto K.,Kitahama K.,Nishimra A.,Jovet A.,Nishi K.,Arai R.,Jovet M.,Nagata I.,: "Tyrosine hydroxylase and aromatic L-amino acid decarboxylase do not coexist in neurons in the human anterior cingulate cortex."Neurosci Lett.. 269. 37-40 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Nagatsu,K.Ikemoto,K.Kitahama,A.Nishimura,H.Ichinose,T.Nagatsu: "Specific localization of the guanoshine triphosphate (GTP) cyclohydrolase I-immunotreactivity in the human brain."J Neuro Transm. 106. 607-617 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Yamamoto,Y-H.Li,I.Ushiyama,A.Nishimura,I.Ohkubo,K.Nishi.: "Puromycin-sensitive alanyl aminopeptidase from human liver cytosol : purificationand characterization."Forensic Science Interational. 113. 143-143 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Ohkubo,Y-H.Li,T.Maeda,Y.Yamamoto,T.Yamane P-G Du,K.Nishi.: "Molecular cloning and immnohistochemical localization of rat dipeptidyl peptidase III"Forensic Science Interational. 113. 147-151 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.Nishimura,S.Sawada,I.Ushiyama,Y.Yamamoto,T.Nakagawa,A.Tanegashima,K.Nishi: "Lectin-histochemical detection of degenerative glycoconjugate deposits in human brain."Forensic Science International. 113. 265-269 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Ikemoto,A.Nishimura,N.Okado,M.Mikuni,K.Nishi,I.Nagatsu: "Human midbrain dopamine neurons express serotonin 2A receptor : an immunohistochemical demonstration."Brain Research. 853. 377-380 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Ikemoto,R.Amano,A.Ishibe,A.Nishimura,K.Nishi,I.Nagatsu: "Quantitative analysis of tyrosine hydroxylase-, aromatic L-amino acid decarboxylase- or phenylethanoamine-N-methyltransferase-immnoreactive neurons in the human medullary C1 region"Acta Histochem.Cytochem.. 33. 259-265 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.Nishimura,S.Sawada,I.Ushiyama,A.Tanegashima,T.Nakagawa,K.Ikemoto,Y.Yamamoto,K.Sato,S.Rand,B.Brinkmann,K.Nishi: "Postmortem diagnosis of brain disorders : The histochemical detection of glycoconjugate deposition with lectins."Anil Aggrawal's Internet J of Forensic Medicine. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 澤田園,西村明儒,牛山郁子,中川季子,山本好男,西克治: "鍍金法と二重の免疫染色による三重染色の法医神経病理学的診断への応用"法医病理. 6. 46-54 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Ohkubo, Y-H.Li, T.Maeda, Y.Yamamoto, T.Yamane, K.Nishi: "Dipeptyidyl peptidase III from rat liver cytosol : purification, molecular cloning and immunohistochemical localization"Bilo.Chem.. 380. 1421-1430 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Ikemoto, K.Kitahama, A.Nishimura, A.Jouvet, K.Nishi, R.Arai, M.Jouvet, I.Nagatsu: "Tyrosine hydroxylase and aromatic L-amino acid decarboxylase do not coexist in neurons in the human anterior cingulate cortex"Neuroscience Letters. 269. 37-40 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Nagatsu, K.Ikemoto, K.Kitahama, A.Nishimura, H.Ichinose, T.Nagatsu: "Specific localization of the guanoshine triphosphate (GTP) cyclohydrolase I-immunotreactivity in the human brain"J Neuro Transm. 106. 607-617 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Yamamoto, Y-H.Li, I.Ushiyama, A.Nishimura, I.Ohkubo, K.Nishi.: "Puromycin-sensitive alanyl aminopeptidase from human liver cytosol : purificationand characterization."Forensic Science Interational. 113. 143-146 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Ohkubo, Y-H.Li, T.Maeda, Y.Yamamoto, T.Yamane P-G Du, K.Nishi.: "Molecular cloning and immnohistochemical localization of rat dipeptidyl peptidase III"Forensic Science Interational. 113. 147-151 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.Nishimura, S.Sawada, I.Ushiyama, Y.Yamamoto, T.Nakagawa, A.Tanegashima, K.Nishi: "Lectin-histochemical detection of degenerative glycoconjugate deposits in human brain."Forensic Science Interational. 113. 265-269 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Ikemoto, A.Nishimura, N.Okado, M.Mikuni, K.Nishi, I.Nagatsu: "Human midbrain dopamine neurons express serotonin 2A receptor : an immunohistochemical demonstration."Brain Research. 853. 377-380 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Ikemoto, R.Amano, A.Ishibe, A.Nishimura, K.Nishi, I.Nagatsu: "Quantitative analysis of tyrosine hydroxylase-, aromatic L-amino acid decarboxylase-or phenylethanoamine-N-methyltransferase-immnoreactive neurons in the human medullary C1 region"Acta Histochem. Cytochem. 33. 259-265 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.Nishimura, S.Sawada, I.Ushiyama, A.Tanegashima, T.Nakagawa, K.Ikemoto, Y.Yamamoto, K.Sato, S.Rand, B.Brinkmann, K.Nishi: "Postomortem diagnosis of brain disorders : The histochemical detection of glycoconjugate deposition with lectins."Anil Aggrawal's Internet J of Forensic Medicine, 1, July 22. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] S.Sawada, A.Nishimura, I.Ushiyama, T Nakagawa, Y.Yamamoto, K.Nishi: "The triple staining method with the silver impregnation and the double immunostain together ; its application to the forensic neuropathological diagnosis."Forensic Pathology. 6. 46-54 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Yamamoto,Y-H. Li,I.Ushiyama,A.Nishimura,I.Ohkubo,K.Nishi.: "Puromycin-sensitive alanyl aminopeptidase from human liver cytosol : purificationand characterization."Forensic Science Interational. 113. 143-146 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] I.Ohkubo,Y-H.Li,T.Maeda,Y.Yamamoto,T.Yamane P-G Du,K.Nishi.: "Molecular cloning and immnohistochemical localization of rat dipeptidyl peptidase III"Forensic Science Interational. 113. 147-151 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Nishimura,S.Sawada,I.Ushiyama,Y.Yamamoto,T.Nakagawa,A.Tanegashima,K.Nishi: "Lectin-histochemical detection of degenerative glycoconjugate deposits in human brain."Forensic Science Interational. 113. 265-269 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Ikemoto,A.Nishimura,N.Okado,M.Mikuni,K.Nishi,I.Nagatsu: "Human midbrain dopamine neurons express serotonin 2A receptor : an immunohistochemical demonstration."Brain Research. 853. 377-380 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Ikemoto,R.Amano,A.Ishibe,A.Nishimura,K.Nishi,I.Nagatsu: "Quantitative analysis of tyrosine hydroxylase-, aromatic L-amino acid decarboxylase- or phenylethanoamine-N-methyltransferase-immnoreactive neurons in the human medullary C1 region."Acta Histochem.Cytochem.. 33. 259-265 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Nishimura,S.Sawada,I.Ushiyama,A.Tanegashima,T.Nakagawa,K.Ikemoto,Y.Yamamoto,K.Sato,S.Rand,B.Brinkmann,K.Nishi: "Postmortem diagnosis of brain disorders : The histochemical detection of glycoconjugate deposition with lectins."Anil Aggrawal's Internet J of Forensic Medicine,. (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 澤田園,西村明儒,牛山郁子,中川季子,山本好男,西克治: "渡金法と二重の免疫染色による三重染色の法医神経病理学的診断への応用"法医病理. 6. 46-54 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 澤田園、西村明儒、牛山郁子、中川季子、山本好男、西克治: "鍍銀法と二種の免疫染色による三重染色の法医神経病理診断学への応用"法医病理. 6巻(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ikemoto K.,Kitahama K.,Nishimura A.,Jovet A.,Nishi K.,Arai R.,Jovet M.,Nagata I.: "Tyrosine hydroxylase and aromatic L-amino acid decarboxylase do not coexist in neurons in the human anterior cingulate cortex"Neurosci Lett.. 269. 37-40 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohkubo I.,Li Y-H.,Maeda T.,Yamamoto Y.,Yamane T.,Nishi K.: "Dipeptidyl peptidase III from rat liver cytosol: Purification, molecular cloning and immunohistochemical localization"Biol. Chem.. 380. 1421-1430 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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