| Project/Area Number |
11670724
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
|
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| Research Institution | Fukuoka University |
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Principal Investigator |
NOMOTO Jyuko School of Medicine, Research Assistant, 医学部, 助手 (50299559)
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| Co-Investigator(Kenkyū-buntansha) |
IDEISHI Munehito School of Medicine, Professor, 医学部, 教授 (20131807)
JIMI Shiro School of Medicine, Research Assistant, 医学部, 助手 (30226360)
SAKU Keijirou School of Medicine, Professor, 医学部, 教授 (40183371)
NODA Keita School of Medicine, Lecturer, 医学部, 講師 (70289536)
KUMAGAI Koichiro School of Medicine, Lecturer, 医学部, 講師 (10248510)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Endothelin / Endothelin type B receptor / antisense oligodeoxynucleotides / Microbubble / dpGapoE / lipofectamine / マイクロバブル法 / エンドセリン-1(ET-1) / 遺伝子多型 / Lys198Asn(K198N)多型 / 冠危険因子 / 動脈硬化症 / 遺伝子治療 / エンドセリン / 血管 / CETP / ベクター |
|---|
| Research Abstract |
Over the past decade, antisense oligodeoxynucleotides (AS-ODNs) have been recognized as a new generation of putative therapeutic agents. The use of ODNs as therapeutic tools requires that they enter target cells and interact with their cellular targets to have a therapeutic effects. However, ODNs are usually unable to enter most cell types with high efficiency. Therefore, some forms of efficient nuclear delivery system is essential.
Here, we use dpGapoE peptide, lipofectamin and ultrasound exposure techniques. We targeted the genes of endothelin type A and B receptors in coronary endothelial cells or smooth muscle cells. Although the percentage of transfected cells by ultrasound with micro bubbles (25%) was lower than that of lipofection (69%) under the fluorescence microscopy, the percentage was comparable to dpGapoE method. This is the first demonstrated use of an ultrasound exposure technique in a nonviral gene delivery system.
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