Project/Area Number |
11671188
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General surgery
|
Research Institution | JIKEI UNIVERSITY SCHOOL OF MEDICINE |
Principal Investigator |
SUZUKI Yutaka JIKEI UNIVERSITY SCHOOL OF MEDICINE SURGERY ASSISTANT, 医学部, 講師 (20241060)
|
Co-Investigator(Kenkyū-buntansha) |
高岡 徹 東京慈恵会医科大学, 医学部, 助手 (30241061)
ISHIBASHI Yoshio JTKEI UNIVERSITY SCHOOL OF MEDICINE SURGERY ASSISTANT, 医学部, 助手 (00246373)
KUBO Hirotaka JIKEI UNIVERSITY SCHOOL OF MEDICINE SURGERY ASSOCIATE PROFESSOR, 医学部, 助教授 (70119791)
|
Project Period (FY) |
1999 – 2000
|
Project Status |
Completed (Fiscal Year 2000)
|
Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
|
Keywords | LH-RH analogue / Tamoxifen / Osteoporosis / LH-RHagonist / 閉経 / 骨障害 / Tamaxifen |
Research Abstract |
The effect of LH-RH analogue or/and Tamoxifen on bone in rats were investigated biochemically and histomorphologically. 12-week old female Wistar rats were in the experiment. The animals were divided into five groups. Specifically, there were sham operation group, ovariectomy group, LH-RH analogue (3.6mg) group, LH-RH analogue(3.6mg), given 0.01mg/kg of Tamoxifen group and LH-RH analogue (3.6mg), given 0.1mg/kg of Tamoxifen group. LH-RH agonist was injected hypodermically. Tamoxifen was given orally to the rats. The evaluation of bone disorders was characterrized by reduction in ash content of the femur and histologically by a disappearance of the trabecular bone in femur metaphysis. The metabolic bone disorders developd in injected LH-RH analogue and ovariectomised rats were osteoporosis histomorphologically. The bone mass in rats after stopped LH-RH analogue was increasing. Tamoxifen could not prevent bone volume loss induced LH-RH analogue.
|