Anti-adhesion Effect of Crosslinked Sodium Hyaluronate Gel.
| Project/Area Number |
11671457
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | JIKEI UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
SAI Shigaku Jikei University School of Medicine, Department of Orthopaedic Surgery, Assistant Professor, 医学部, 助教授 (80183359)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Tukasa Jikei University School of Medicine, Department of Orthopaedic Surgery, Research Assistant, 医学部, 助手 (90287243)
TANAKA Takaaki Jikei University School of Medicine, Department of Orthopaedic Surgery, Lecturer, 医学部, 講師 (80171768)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Cross-linked hyaluronatehvdrogel / Cell adhesion / Adhesion / Joint surgery |
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| Research Abstract |
Functional disturbances resulting from adhesion formation after tendon injury present one of the most difficult problems associated with this type of injury. Since previous studies showed that hyaluronic acid (HA) reduced adhesions after flexor tendon injury, we. attempted to minimize tendon adhesions by using cross-linked hyaluronate hydrogel and photocured with ultraviolet irradiation.
The flexor tendons of the right third and fourth hind limb toes Of 30 rabbits were used in the present. study, and three tendon adhesion factors were elicited, namely, injury to the tendon, injury to the tendon sheath, and injury ..to the gliding floor. Standard adhesions were produced by all factors in addition to cast immobilization , and this surgical procedure. was usedto model standard adhesions (control group) in the following experiments. In the experimental group, cross-linked hyaluronate .hydrogel was inserted between the tendon and bone immediately after the injury procedure (HA group). The in
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jured site was then immobilized in . a cast for three weeks, and rabbits were sacrificed at three weeks after operation. The severity of tendon adhesions was compared by macroscopic and microscopic examination, by testing the mobility of the digits, and by the pulling-out weight of the tendon against adhesion. All specimens were stained with hematoxylin and eosin for microscopic examination.
Macroscopically, less adhesion was observed between the gliding floor and the tendon in the HA group than in the control group. Microscopically, the tendon was found to be adherent to the surrounding granulation tissue in the control group both three weeks after the operation, whereas there was significantly less granulation tissue between the tendon and bone in the HA group. The range of motion of the distal interphalangeal joint measured by pulling the flexor digitorum profundus tendon was greater in the HA group than in the control group, and the pulling-out weight was lower in the HA group than in the control group. Thus, the data as a whole indicate that cross-linked hyaluronate hydrogel could be applied clinically to prevent adhesions after tendon injury. Less
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Report
(4 results)
Research Products
(6 results)
