Gene transfection in human keratinocyte and melanocyte
| Project/Area Number |
11671780
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YAMAMOTO Yuhei Hokkaido Univ., Medical Hospital, Assistant Prof., 医学部・附属病院, 助手 (70271674)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIHARA Tsuneki Hokkaido Univ., Grad.School of Med., Chief Prof., 大学院・医学研究科, 教授 (20002157)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | transfection / ha-tagged vector / transfectant / lipofection / electroporation / gelsolin / gelsolin truncate / human primary melanoma / マーカー遺伝子 / 導入効率 / ヒト表皮角化細胞 / ヒトメラノサイト系細胞 |
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| Research Abstract |
1 At first, we studied the transfection of ha-tagged human gelsolin cDNA in human squamous carcinomacell line HEC46 and human melanoma cell line in stead of keratinocyte and melanocyte. We used human melanoma tissue and normal skin tissue as a control, and found out a unique 85Kda protein in Western blotting.
2 By Western blotting analysis of 38 melanoma samples for the expression of gelsolin using anti-gelsolin antibodies, we identified a new truncated variant of gelsolin p85 (GSNp85) lacking the C-terminal domain, which was always co-expressed with wild type gelsolin. The GSNp85 variant was more frequently expressed in vertical growth phase (VGP) melanomas as compared to radial growth phase (RGP) melanomas and was not expressed in other skin cancers, normal skin tissues, and nevi tissues except for 2 congenital junctional types. These data suggest that the occurrence of GSNp85 in melanoma may be a new indicator for the advanced stage of the tumor.
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Report
(3 results)
Research Products
(3 results)
