| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
Sodium fluoride (5.26 mM = 100 ppm F), in combination with component of jacks or ordinary medicament (caffeine, ethanol, nicotine, aspirine, warfatine) or under some specific disease conditions (osteoporosis, glomerulonephritis, diabetes mellitus), was orally given to 6-week old male mouse from drinking water for 10 days, and then, drinking water intake, body weight gain, blood fluoride concentration, non-specific esterase and chloroacetate esterase expression of bone marrow cells, cell surface antigen Mac-1, Gr-1, MOMA-2, F4/80 expressions, and, cell viability, NBT reduction test, NO generation by LPS stimulation (NO_2 production), LDH, β-glucuronidase, acid phosphatase (ACP) activities, phagocytic ability, detached/adherent cell number ratio, nucleic/cytoplasmic areal ratio, or microscopic appearance were examined. Though blood fluoride concentrations were significantly elevated by sodium fluoride from drinking water, none of other markers except LDH, β-glucuronidase, ACP activities was conclusively changed, however, the values of those markers were fluctuated under those specific disease conditions.
When bone marrow cells of 8-week old male mouse were cultured in the presence of NaF in combination with 1, 25-dihydroxyvitamin D_3, Mac-1, Gr- 1 and chloroacetate esterase were dose-dependentiy up-regulated with NaF concentration but not with 1, 25-dihydroxyvitamin D_3 concentration and non-specific esterase was not influenced. Cell viability and NBT reduction ability were incompetently suppressed at 0.5 mM NaF and NO generation, or LDH, β-glucuronidase, ACP activities was maximized at 0.2 or 0.3 mM but phagocytic ability, detached/adherent cell number ratio, nucleic/cytoplasmic areal ratio, or microscopic appearance was almost not changed.
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