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In vitro preservation of medicinal plants

Research Project

Project/Area Number 11672232
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionNiigata University of Pharmacy and Applied Life Science

Principal Investigator

HIRAOKA Noboru  Niigata University of Pharmacy and Applied Life Science, Faculty of Pharmacy, Ph.D., Professor, 薬学部, 教授 (00025701)

Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsplant tissue culture / medicinal plant / germplasm preservation / cold storage / Atractylodes / Scopolia japonica / essential oil / tropane alkaloid / 遺伝子源保存 / レモングラス / チョウセンアサガオ / シュート培養 / ハマボウフウ / フラノクマリン / ポリアセチレン化合物
Research Abstract

The present research project was successful in micropropagation protocol and medium term preservation method for in vitro shoot culture of Scopolia japonica Maxim. (Solanaceae) and Atractylodes ovata DC. (Compositae). In vitro grown shoots were successfully stored at 2℃ up to 2 years. Reculturing of the shoots in freshly prepared Linsmaier and Skoog medium supplemented with indole-3-acetic acid (1μM) and 6-benzyladenine (10μM) showed hundred percentage viability. Excised shoots were rooted in LS medium containing none of plant growth regulators or indole-3-acetic acid (1μM).
Three- and four-year-old in vitro raised plants of S.japonica and the regenerated plants of A.lancea cultivated in the field for one or two years did not show any detectable variability in their morphological as well as biochemical characters, i.e. the composition and contents of tropane alkaloids in the S.japonica rhizome and the contents of atractylon, a sesquiterpene component of essential oil in the A.lancea rhizome, respectively, when compared with the each control set.
Results reveals that non-frozen cold storage of in vitro shoot cultures of medicinal plants can be used as a germplasm preservation system for a medium-duration without deterioration of their biochemical as well as biological characteristics.

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

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