Activity-dependent circuit formation in the neocortex
| Project/Area Number |
11680788
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Osaka University |
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Principal Investigator |
YAMAMOTO Nobuhiko Osaka University, Graduate School of Engineering Science, Associate Professor, 大学院・基礎工学研究科, 助教授 (00191429)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | neocortex / horizontal axon / neuronal activity / organotypic culture / branching / synaptic transmission / rat / 大脳皮質 / 分枝形成 / 神経活動依存性 / タイムラプス観察 / スライス培養 |
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| Research Abstract |
In the developing visual cortex axons from upper layer cells travel along the same layers and form branches and synaptic connections. To date morphological and physiological studies have indicated that the formation of the horizontal connections is dependent on neuronal activity. However, how electrical activity influences branching of individual axons is not known. In addition, the molecular mechanisms underlying this process are almost unknown. We address this issue by using organotypic culture preparations of cortical slices. A new axon tracing method in which a small fraction of neurons are labeled with GFP was also developed. These methods enable us to observe individual axon arbors for a long period.
In this study we have obatined the following results. First, axons from cortical cells in the upper layers formed branches in vitro in a fashion which is similar to that in vivo. Second, branching was accompanied by growth and retraction, suggesting that branch formation is a dynamic process. Third, branching was down-regulated when overall neuronal activity was suppressed by pharmacological treatments. Finally, the result suggests that RhoA, a small GTPase, is involved in intracellular mechanisms which convert electrical signals to the axon morphology.
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Report
(3 results)
Research Products
(13 results)
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[Publications] Yamamoto, N., Matsuyama, Y., Harada, A., Inui, K., Murakami, F.and Hanamura, K: "Characterization of factors regulating lamina-specific growth of thalamocortical axon."J.Neurobiol.. 42. 56-68 (2000)
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[Publications] Yamamoto, N., Inui, K., Matsuyama, Y., Harada, A., Hanamura, K., Murakami, F., Ruthazer, E.S., Rutishauser, U.and Seki, T.: "Inhibitory Mechanism by Polysialic Acid for Lamina-Specific Branch Formation of Thalamocortical Axons."J Neurosci. 20. 9145-9151 (2000)
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