| Project/Area Number |
11680791
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
OZAWA Hitoshi Kyoto Prefectural University of Medicine, Department of Anatomy & Neurobiology, Associate professor, 医学部, 助教授 (60169290)
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| Co-Investigator(Kenkyū-buntansha) |
ARII Tatsuo National Institute for Physiological Science, Center for Brain Experiment, 生理学研究所, 助教授 (10109267)
MORITA Noriyuki Kyoto Prefectural University of Medicine, Department of Anatomy & Neuobiology, Assistant professor, 医学部, 講師 (50239662)
NISHI Mayumi Kyoto Prefectural University of Medicine, Department of Anatomy & Neurobiology, Assistant professor, 医学部, 講師 (40295639)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Aging / Glucocorticoid receptor / Steroid hormone / Immunohistochemistry / Ultra structure / High voltage electron microscopy / Neuroendocrine / Neurotransmitter / ステロイドホルモン受容体 / ラット / グルココルチコイド / アネキシン1 / 転写調節 / 共役転写因子 / 免疫電子顕微鏡 |
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| Research Abstract |
We immunohistochemically investigated the GR expression in the brain of aged-rats by means of. In comparison with young adult rats, the reduction of intensity for GR-immunoreactivity was observed in the cerebral cortex, hippocampus and amygdala, which were well associated to the limbic system, in the aged-rats, although the circulating cortiosteroids have been indicated to show an increase with aging.
The ultra structural changes on the neurons with aging, especially using a high voltage electron microscopy (HVEM) was also observed. HVEM could reveal the significant reduction of the number of spines in dendrites of CA1 pyramidal neurons and cortical neurons, and the morphological changes of these spines. On the other hand, we immunohistochemically investigated the change of several neurotransmitter relevant enzymes, such as ChAT, GAD, and TH. In the aged rats, the reduction of immunoreactivity for ChAT, as well as GAD were observed in comparison with those in the young rats. On the othe
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r hand, the intensity of TH immunoreactivity did not show any significant changes in aged rats, although the number of TH-immunoreactive cell bodies and fibers seemed to reduce.
Moreover, we studied about the expression of annexin-1 in the folliculo-stellate cells in the anterior pituitary under different corticosteroid conditions. After adrenalectomy, LC1 immunoreactivity was almost disappeared, while the immunoreactivity was recovered by corticosterone replacement. The expression of GR immunoreactivity in the nucleus of FS cells also showed a similar pattern to annexin-1 associated with the corticosteroids conditions, however S100 (a marker for FS cells) immunoreactivity was not changed whatever corticosteroids conditions.
These results suggest that glucocorticoids regulate the LC1 expression and promote the translocation of annexin-1 from intracellular to pericellular sites in the FS cells of rat anterior pituitary gland to interact with neighboring endocrine cells as a paracrine action. The change of annexin-1 expression in the aged rat is now going to investigate. Less
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