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Functional analysis of Lbx1 in muscles of chimeric mice

Research Project

Project/Area Number 11680809
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionKitasato University

Principal Investigator

HANAOKA Kazunori  Kitasato University, School of Science, Professor, 理学部, 教授 (40189577)

Co-Investigator(Kenkyū-buntansha) UCHIYAMA Koji  Kitasato University, School of Science, Assistant, 理学部, 助手 (20276174)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsLbx1 / chimeric mice / myogenesis / skeletal muscle / myoblasts / cell migration / muscular dystrophy / homeobox
Research Abstract

Lbx1, a mouse homeobox gene, is expressed in migratory myoblasts that form musculature of limb, diaphragm, and tongue but not expressed in non-migratory myoblasts in the myotomes at the trunk level. This specific expression patterns suggest that Lbx1 may play a key role in specification and differentiation of migratory myoblasts. To clarify the function of Lbx1, we generated chimeric mice using LacZ labelled-ES cells carrying Lbxl under the control of myogenin promoter. In such chimeras, we did not detect any abnormal behavior of migrating myoblasts during embryogenesis. Two weeks after birth, however, these chimeric mice exhibited obvious morphological abnormalities in limb and back muscles. Irregularity in the sizes of the muscle fibers with presence of giant fibers, centrally placed nuclei, whorled fibers, necrosis and phagocytosis, all of which are known as signs of muscle degeneration-regeneration occurring, were observed. These impaired muscles were found to be always contributed with the ES-derived cells, and the extent of damage was in proportion to the contribution rate of the ES-derived cells. Here, we demonstrate interesting phenotypes of the chimeric mice, which may be a cue to understand how Lbx1 functions during myogenesis.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Mohri K,Takano-Ohmuro H,Nakashima H,Hayakawa K,Endo T,Hanaoka K,Obinata T: "Expression of cofilin Isoforms during development of Mouse Striated Muscle"J Muscle Res Cell Motil. 21(1). 49-57 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Uchiyama K.,Ishikawa A.and Hanaoka K.: "Expression of Lbxl involved in the hypaxial musculature formation of the mouse embryo."J Exp Zool. 286(3). 270-279 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Uchiyama,R.Ohtuka and Hanaoka,K:: "cHox1 1L2,a Hox11 related gene, is expressed in the peripheral nervous system and subpopulation of spinal cord during chick development"Neurosci.Lett. 273(2). 97-100 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kameya S,Miyagoe Y,Nonaka I,Ikemoto T et al: "alphal-syntrophin gene disruption results in the absence of neuronal-type nitric oxide synthetase at the salcolemma, but does not induce muscle degeneration."J.Biol Chem.. 274(4). 2192-2200 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kameya S, Miyagoe Y, Nonaka I, Ikemoto T, Endo M, Hanaoka K, Nabeshima Y and Takeda S: "alphal-syntrophin gene disruption results in the absence of neuronal-type nitric oxide synthetase at the salcolemma, but does not induce muscle degeneration."J Biol Chem. 274, No4. 2193-2200 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Uchiyama, R.Ohtuka and Hanaoka, K: "cHox11L2, a Hox11 related gene, is expressed in the peripheral nervous system and subpopulation of spinal cord during developments"Neurosci Lett Oct. 273 No2. 97-100 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Uchiyama K., Ishikawa A.and Hanaoka K: "Expression of Lbxl involved in the hypaxial musculature formation of the mouse embryo"J Exp Zool. 286 No3. 270-9 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mohri K, Takano-Ohmuro H, Nakashima H, Hayakawa K, Endo T, Hanaoka K, Obinata T: "Expression of cofilin Isoforms during development of Mouse Striated Muscle"J Muscle Res Cell Motil. 21 No1. 49-57 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mohri K et al: "Expression of cofilin Isoforms during development of Mouse Striated Muscle"J Mouscle Research and Cell Motility. 21. 49-57 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Uchiyama K..Ishikawa A.and Hanaoka K.: "Expression of Lbx1 involved in the hypaxial musculature formation of the mouse embryo."J Exp Zool. 286. 270-279 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Uchiyama,R.Ohtuka and Hanaoka,K :: "cHox11L2, a Hox11 related gene, is expressed in the peripricral nervous system and subpopulation of spinal cord during chick development"Neurosci.Lett. 273.(2). 97-100 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mohri K et al: "Expression of cofilin Isoforms during development of Mouse Striated Muscle"J Mouscle Research and Cell Motility. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] Uchiyama K., Ishikawa A. and Hanaoka K.: "Expression of Lbxl involved in the hypaxial musculature formation of the mouse embryo."J Exp Zool. (印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Uchiyama, R .Ohtuka and Hanaoka, K:: "cHox11L2, a Hox11 related gene, is expressed in the periprieral nervous system and subpopulation of spinal cord during chick development"Neurosci. Lett. (印刷中).

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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