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Mutations of the core gene sequence of HCV from patients with hepatocellular carcinoma in China and Japan

Research Project

Project/Area Number 11695087
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionUniversity of Tokushima

Principal Investigator

ITO Susumu  University of Tokushima, School of Medicine, Professor, 医学部, 教授 (70093838)

Co-Investigator(Kenkyū-buntansha) KIDO Hiroshi  University of Tokushima, Institute for Enzyme Research, Professor, 分子酵素学研究センター, 教授 (50144978)
SHIMIZU Ishiro  University of Tokushima, School of Medicine, Instructor, 医学部・附属病院, 講師 (10178965)
孟 せんよう  中華人民共和国, 南通医学院, 教授
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsHepatocellular Carcinoma / Hepatitis C Virus / Clustering Variable Region / Amino Acid Alteration / Nucleotide Substitution
Research Abstract

Firstly, we investigated the association between hepatocellular carcinoma(HCC)and the genomic characteristics of the hepatitis C virus(HCV)isolated from residents of the inshore region of the Yangtze River. That area of China has one of the highest incidences of HCC.Our data revealed that nucleotide substitutions are unevenly scattered along the HCV genome, with a cluster of missense mutations in the region encoding the second hydrophilic domain of the core protein, and that the number of these mutations in the clustering variable region(CVR)is significantly higher in isolates from HCC patients than in those from individuals with chronic hepatitis. Secondly, we determined the sequence divergence of the CVR in HCV isolates from cancerous and noncancerous liver tissues in Japanese patients with HCC.The number of nucleotide substitutions giving rise to changes in amino acid residues in the CVR was significantly greater in liver tissues than in sera from HCC patients. The number of amino acid residues in the CVR that differed from the representative clone from cancerous liver portions was significantly higher than the number differing from noncancerous portions in each patient. An amino acid alteration from Gly to Ser at core codon 45 in the CVR was dominant in noncancerous portions rather than in cancerous portions and sera from HCC patients. These findings suggest that a large number of mutations, including biologically important amino acid sequence changes in the HCV core gene from liver tissue, might be related to hepatocarcinogenesis.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Horie T, Shimizu I, et al.: "Mutations of the core gene sequence of hepatitis C virus isolated from liver tissues with hepatocellular carcinoma"Hepatol Res. 13(3). 240-251 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 清水一郎: "肝炎ウイルスと肝癌"医薬ジャーナル. 35(6). 77-82 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Omoya Y, Shimizu I, et al.: "Mutations in a hydrophilic part of the core gene of hepatitis C virus from patients with hepatocellular carcinoma in China. In ; Proceeding of 3rd International Conference on Therapies for Viral Hepatitis"(1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shimizu I, Ito S, et al.: "Mutations of the core gene sequence of HCV from patients with hepatocellular carcinoma in China and Japan. In ; Proceeding of 7th International Hepatitis C Virus & Related Disease(Molecular Virology and Pathogenesis)"(2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Omoya T, Shimizu I, et al.: "Mutations of the core gene sequence of HCV from patients with hepatocellular carcinoma in China. In ; Frontiers in Viral Hepatitis.Eds.Schinazi R, Rice C, Sommadossi JP."International Medical Press(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Horie T, Shimizu I, Horie C, Yogita S, Tashiro S, Ito S: "Mutations of the core gene sequence of hepatitis C virus isolated from liver tissues with hepatocellular carcinoma."Hepatol Res. 13(3). 240-251 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Omoya Y, Shimizu I, Yao D-F, Honda H, Meng X-Y, Ito S: "Mutations in a hydrophilic part of the core gene of hepatitis C virus from patients with hepatocellular carcinoma in China"In ; Proceeding of 3rd International Conference on Therapies for Viral Hepatitis. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shimizu I, Inoue H, Itonaga M, Lu G, Zhou Y, Horie T, Ito S: "Mutations of the core gene sequence of HCV from patients with hepatocellular carcinoma in China and Japan."In ; Proceeding of 7th International Hepatitis C Virus & Related Disease(Molecular Virology and Pathogenesis). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Omoya T, Shimizu I, Horie C, Horie T, Yao D-F, Itonaga M, Okamura Y, Honda H, Ito S: "Mutations of the core gene sequence of HCV from patients with hepatocellular carcinoma in China."In ; Frontiers in Viral Hepatitis. Eds.Schinazi R, Rice C, Sommadossi JP, International Medical Press. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shimizu I,et al.: "Mutations of the core gene sequence of HCV from patients with hepatocellular carcinoma in China and Japan (Proceeding)"117 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Omoya T,et al.: "Mutations of the core gene sequence of HCV from patients with hepatocellular carcinoma in China. In; Frontiers in Viral Hepatitis. Eds. Schinazi R, Rice C, Sommadossi JP."International Medical Press (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] HorieT,et al.: "Mutations of the core gene sequence of hepatitis C virus isolated from liver tissues with hepatocellular carcinoma"Hepatology Research. 13. 240-251 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 清水一郎、他: "肝炎ウイルスと肝癌"医薬ジャーナル. 35(6). 1509-1514 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] OmoyaT,et al.: 3rd International Conference on Therapies for Viral Hepatitis(Proceeding). (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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