Molecularpathological investigation of familial canine epilepsy as a use for human familial frontal lobe epilepsy.
| Project/Area Number |
11836005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | Tottori University |
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Principal Investigator |
MORITA Takehito Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70273901)
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| Co-Investigator(Kenkyū-buntansha) |
HIKASA Yoshiaki Faculty of Agriculture, Professor, 農学部, 教授 (30165071)
SERIKAWA Tadao Faculty of Agriculture, Professor, 医学部, 教授 (30025655)
SHIMADA Akinori Faculty of Agriculture, Professor, 農学部, 教授 (20216055)
SATOH Kota Faculty of Agriculture, Research Assistant, 農学部, 助手 (50283974)
TAKEUCHI Takashi Faculty of Agriculture, Research Assistant, 農学部, 助手 (20216849)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | electroencephalography / epilepsy / familial / dog / 遺伝 |
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| Research Abstract |
We determined an epileptic focus by electroencephalography using an international 10-20 electrode system in 11 Shetland sheep dogs affected with famailial idiopathic epilepsy and performed evaluation of amino acids in the cerebrospinal fluid (CSF) and pathologic examination of the brain in 8 dogs died from status epilepticus. Continous electroencephalography (EEG) demonstrated that an epileptic focus was initially detected in the frontal lobe and that paroxysmal foci developed diffusely in other lobes of affected dogs with recurrent convulsions. EEG analysis indicated spike and sharp wave complexes which were considered to be paroxysmal discharges. A significantly high value of threonine was found in the CSF of epileptic dogs. Histologically, acute neuronal necrosis and astrocytosis were distributed predominantly in the cingulate cortex and internal area of frontal cortex, less frequently in other areas of the cerebrum. Immunohistochemical study using anti-glutamate transporter antibody showed that its weak expression was found in the cerebrum comparing of the control dog. The results of this study suggest that the dogs have initially an epileptic focus in the frontal lobe, and that the focus extends gradually to other areas of the cerebrum. Based on the distribution of neuronal necrosis and astrocytosis, acute neuronal damage may be related with superexcitation (due probably to glutamate) of neurons following epilepsy.
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Report
(3 results)
Research Products
(3 results)
