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Study on the effects of endocrine disruptors on development and differentiation of gonads and central nervous system.

Research Project

Project/Area Number 11839004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

WATANABE Gen  Faculty of Agriculture, Tokyo University of Agriculture and Technology Associate Professor, 農学部, 助教授 (90158626)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsendocrine disruptor / octylphenol / estrogen / LHRH / sexual differentiation / inhibin / オクチルフェノール
Research Abstract

We have investigated mainly about the effects of endocrine disruptors on sexual differentiation of CNS in rats.
1) LHRH secretion during neonatal period is critical for normal development of male sexual function in hypothalamo-pituitary-gonadal axis in rats. Some of endocrine disruptors are known to have estrogenic action and be able to suppress LHRH secretion. We evaluate the effects of LHRH suppression on the fertility of male rats by injecting anti-LHRH antiserum raised in goats during neonatal of male rats.
In the results, male rats received LHRH antiserum for six days after birth showed normal sperm production in their testes. When they were challenged with estrous females, they showed little mating behavior and showed no copulation. Areas of the sexual dimorphic nucleus of preoptic area in the anti-LHRH treated males seemed smaller than those of normal males and a little larger than normal females. In conclusion, LHRH suppression during the critical period of sexual differentiation in central nervous system would result in the impotence of males at adulthood.
2) We injected s.c. an estrogenic endocrine disruptor, octylphenol, to neonatal female rats from day 0 to day 5 after birth. After growing to adult, they were ovariectomized and injected estradiol-17β to see the occurrence of gonadotropin surges. Octylphenol treated females did not show LH surge at the evening after estradiol injection. When they were challenged with mature normal male rats, both the lordosis quotient and its intensity were significantly low in octylphenol treated females.
The areas of the sexual dimorphic nucleus in the preoptic area were larger than those od normal female rats.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Shin-ichi Katsuda: "Dose-and treatment duration-related effects of p-tert-octylphenol on female rats"Reproductive Toxicology. 14. 119-126 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chandana B.Herath: "Exposure of neonatal female rats to p-tert-octylphenol disrupts afternoon surges of luteinizing hormone, follide-stimulating hormone…"Biology of Reproduction. 64. 1216-1224 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akiko Takenaka: "Sexual dysfunction induced by neonatal administration of anti-LHRH serum in male rats."International Journal of Urology. 7suppl.. S61 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shin-ichi Katsuda et al.: "Dose-and treatment duration-related effects of p-tart-octylphenol on female rats."Reproductive Toxicology. 14. 119-126 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Chandana B.Herath et al.: "Exposure of neonatal female rats to p-tart-octylphenol disrupts afternoon surges of luteinizing hormone, follicle-stimulating hormone and prolactin secretion, and interferes with sexual receptive behavior in adulthood."Biology of Reproduction. 64. 1216-1224 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akiko Takenaka et al.: "Sexual dysfunction induced by neonatal administration of anti-LHRH serum in male rats."International Journal of Urology. 7 suppl.. s61 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shin-ichi Katsuda: "Dose-and treatment duration-related effects of P-tert-octylphenol on female rats"Reproductive Toxicology. 14. 119-126 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Chandana B.Herath: "Exposure of neonatal female rats to P-tert-octylphenol disrupts afternoon surges of luteinizing hormone, follide-stimulating hormone..."Biology of Reproduction. 64. 1216-1224 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akiko Takenaka: "Sexual dysfunction induced by neonatal administration of anti-LHRH serum in male rats."International Journal of Urology. 7suppl.. S61 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K Ohshima: "Secretion of inhibin A, inhibin B and inhibin pro-αC during the oestrous cycle of the golden hamster (Mesocricetus auratus)"Journal of Endocrinology. 162. 451-456 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H Kishi: "Regulation of gonadotropin secretion by circulating inhibin, estradiol, and progesterone in cyclic hamsters"American Journal Physiology (Endocrinology and Metabolism). 277. E876-E882 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Pei Xin Yang: "Effects of third ventricle injection of norepinephrine analogue on LH secretion of the non-laying SlJI goose"Endocrine Journal. 47(in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Kishi: "Inhibin is an important factor in the regulation of FSH secretion in the adult male hamster"American Journal Physiology (Endocrinology and Metabolism). 278(in press). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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