心臓型アデニル酸シクラーゼとＥｐａｃを治療標的にした新しいベータ遮断薬の開発

• Project/Area Number: 11F01418
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 外国
• Research Field: Circulatory organs internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: 石川 義弘 横浜市立大学, 医学研究科, 教授
• Co-Investigator(Kenkyū-buntansha): 金 美花 横浜市立大学, 医学研究科, 外国人特別研究員
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 2013: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 2012: ¥800,000 (Direct Cost: ¥800,000)
• Keywords: 不整脈 / cAMP / 心臓 / 誘発条件検討 / 誘発条件

Research Abstract

cAMP産生酵素であるアデニル酸シクラーゼは、神経伝達物質をはじめとして各種ホルモン受容体の標的酵素として、細胞内においてセカンドメッセンジャーシグナルを調節する酵素である。心臓のカテコラミン受容体の主体をなすβ受容体の阻害剤であるβ遮断剤は、心臓での交感神経刺激を抑制する薬剤として、心不全や不整脈の治療薬として多方面で使用されている。しかしながら肺や中枢にも発現するために、肺気腫や気管支ぜんそく患者においては、使用禁忌である。そこで心臓のみβ遮断剤が作用するようにすれば、この副作用は軽減される。アデニル酸シクラーゼの多数のサブタイプのうち、心臓型と呼ばれる5型サブタイプは、心臓特異的に発現することが知られている。従ってこの5型サブタイプを選択的に抑制することが出来れば、そあ下流因子のシグナルも含めて抑制できる。本研究では、5型サブタイプの選択抑制が、下流の酵素シグナルであるEpac系の抑制変化を起こしうるかを、遺伝子操作動物との関連において検討した。このためにマウスの心房ページングを、食道ペーシングにおいて施行するモデルを作成し、心臓不整脈の亢進モデルを確立し、これまでの短時間モデルの欠点を克服することを目的とした。電極カテーテルを用いて様々な誘発特性の最適化をおこなった。条件の検討においては、マウスの管理状態、付随する条件検討を中心に行い、カテコラミン誘発性刺激を負荷したところ、ノルアドレナリンの刺激を事前に加えることにより、マウスの不整脈の誘発が亢進し、持続時間が亢進することが解った。この変化は、細胞内のセカンドメッセンジャーシグナルの調節によって変化することが解明された。我々の検討結果から、マウスの不整脈の性状は、・副交感および交感神経刺激の状態によって大きく作用される可能性が示唆され、不整脈の誘発には重要であることが解った。

Strategy for Future Research Activity

(抄録なし)

Research Products

• [Journal Article] Prostaglandin E2 Inhibits Elastogenesis in the Ductus Arteriosus via EP4 Signaling. (2014)
  • Author(s): Yokoyama U, Minamisawa S, Sh ioda A, Ishiwata R, Jin MH, Masuda M, Asou T, Sugimoto Y, Aoki II, Nakamura T, and Ishikawa Y
  • Journal Title: Circulation. Volume: 129 Issue: 4 Pages: 487-496
  • DOI: 10.1161/circulationaha.113.004726
  • Peer Reviewed / Open Access
• [Journal Article] Three-Dimensional Multilayers of Smooth Muscle Cells as a New Experimental Model for Vascular Elastic Fiber Formation Studies. (2014)
  • Author(s): Ishiwata R, Yokoyama U, Matsusaki M, Yoshiya A, Kadowaki K, Ichikaww Y, Umemura M, Fujita T, Minamisawa S, Shimoda H, Mitsuru Akashi M, and Ishikawa Y.
  • Journal Title: Atherosclerosis. Volume: 233 Issue: 2 Pages: 590-600
  • DOI: 10.1016/j.atherosclerosis.2014.01.045
  • Peer Reviewed / Open Access
• [Journal Article] Store-operated Ca2 entry (SOCE) Regulates Melanoma Proliferation and Cell Migration. (2014)
  • Author(s): Umemura M, Baljinnyam E, Feske S, De Lorenzo MS, Lai-Hua Xie LH, Feng X, 1, Itoh K, Makino A, Fujita T, Yokoyama U, Iw atsubo M, Chen S, Goydos JS, I shikawa Y, and Iwatsubo K
  • Journal Title: PLoS ONE. Volume: 9 Issue: 2 Pages: e89292-e89292
  • DOI: 10.1371/journal.pone.0089292
  • Peer Reviewed / Open Access
• [Journal Article] Protection of cardiomyocytes from the hypoxia-mediated injury by a peptide targeting the activator of G-protein signaling 8. (2014)
  • Author(s): Sato M, Hiraoka M, Suzuki H, Sakima M, Mamun, AA, Yamane Y, Fujita T, Yokoyama U, Okumura S, and Ishikawa Y.
  • Journal Title: PLoS ONE. Volume: 9 Issue: 3 Pages: e91980-e91980
  • DOI: 10.1371/journal.pone.0091980
  • Peer Reviewed / Open Access
• [Journal Article] Hyperthermia generated with ferucarbotran (Resovis®) in an alternating magnetic field enhances cisplatin-induced apoptosis of cultured human oral cancer cells. (2014)
  • Author(s): Sato I, Umemura M, Mitsudo K, Kioi M, Nakashima H, Iwai T Fujita T, Yokoyama U, Okumura S, Feng X, Ito K, Miyajima A, Makino A, Iwai M, Eguchi H, I wai T, and Ishikawa Y.
  • Journal Title: J. Physiol. Sci. Volume: 64 Issue: 3 Pages: 177-183
  • DOI: 10.1007/s12576-014-0309-8
  • Peer Reviewed / Open Access
• [Journal Article] Geranylgeranylacetone protects the heart via caveloae and caveolin-3. (2014)
  • Author(s): Tsutsumi YM, Tsutsumi R, Hirokawa YT, Sakai Y, Hamaguchi E, Ishikawa Y, Yokoyama U, Kasai A, Kambe N, and Tanaka K.
  • Journal Title: Life Sciences. Volume: 101 Issue: 1-2 Pages: 43-48
  • DOI: 10.1016/j.lfs.2014.02.019
  • Peer Reviewed / Open Access
• [Journal Article] Disruption of Epacl decreasesphosphorylation of phospholamban and protects theheart against stresses. (2014)
  • Author(s): Okumura S, Fujita T, Cai W, Jin M, Namekata I, Mototani Y, Jin HL, Ohnuki Y, Tsuneoka Y, Kurotani R, Suita Km Kawakami Y, Hamaguchi S, Abe T, Kiyonari H, Tsunematsu T, Bai Y, Suzuki S, Hidaka Y, Umemura M, Ichikawa Y, Yokoyama U, Sato M, Ishikawa F, Izumi-Nakaseko H, Adachi-Akahane S, Tanaka H, and Ishikawa Y.
  • Journal Title: J. Clin. Invest. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Epacl increases migration of endothelial cells and melanoma cells via FGF2-mediated paracrine signaling. (2014)
  • Author(s): Baljinnyam E, Umemura M, C huang C, De Lorenzo M, Iwatsubo M, Chen S, Goydos J, Ishikawa Y, Whitelock J, and Iwatsubo K.
  • Journal Title: Pigment Cell & Melanoma Research. Volume: (Epub ahead of print) Issue: 4 Pages: 611-620
  • DOI: 10.1111/pcmr.12250
  • Peer Reviewed
• [Journal Article] The prostanoid EP4 receptor and its signaling pathway. (2013)
  • Author(s): Yokoyama U, Iwatsubo K, Umemura M, Fujita T, and Ishikawa Y.
  • Journal Title: Pharmacol Rev. Volume: 65 Issue: 3 Pages: 1010-1052
  • DOI: 10.1124/pr.112.007195
  • Peer Reviewed
• [Journal Article] Type 5 Adenylyl Cyclase Increases Oxidative Stress by Transcriptional Regulation of MnSOD via the Sirtl/FoxO3a Pathway. (2013)
  • Author(s): Lai L, et al.
  • Journal Title: Circulation. Volume: 127 Issue: 16 Pages: 1692-1701
  • DOI: 10.1161/circulationaha.112.001212
  • Peer Reviewed
• [Journal Article] A role for Piezo2 in EPAC1-dependent mechanical allodynia. (2013)
  • Author(s): Eijkelkamp N, Linley JE, Torres JM, Bee L, Dickenson AH, Gringhuis M, Minett MS, Hong GS, Lee E, Oh U, Ishikawa Y, Zwartkuis FJ, Cox JJ, and Wood, J N.
  • Journal Title: Nature Commun. Volume: 4 Issue: 1 Pages: 1682-1682
  • DOI: 10.1038/ncomms2673
  • Peer Reviewed
• [Journal Article] Hight-dose zoledronic acid narrows the periodontal space in rats. (2013)
  • Author(s): Okamoto Y, Hirota M, Monden Y, Murata S, Koyama C, Mitsudo K, Iwai T, Ishikawa Y, and Tohnai I.
  • Journal Title: Int J Oral Maxillofac Surg. Volume: 2 Issue: 5 Pages: 627-631
  • DOI: 10.1016/j.ijom.2012.11.011
  • Peer Reviewed
• [Journal Article] Balancing GRK2/ Epac1levels prevents and relieves chronic pain. (2013)
  • Author(s): Wang H, Heijnen CJ, van Velth oven CTJ, Willemen HLDM, Ishikawa Y, Zhang X, Sood AK, Vroon A, Eijkelkamp N, and Kavelaars A.
  • Journal Title: J. Clin. Invest. Volume: 123 Issue: 12 Pages: 5023-5034
  • DOI: 10.1172/jci66241
  • Peer Reviewed
• [Journal Article] Adenylyl cyclase type 5 in cardiac disease, metabolism and aging. (2013)
  • Author(s): Vatner SF, Park M, Yan L, Lee G, Lai L, Iwatsubo K, Ishikawa Y, Pessin J, and Vatner DE
  • Journal Title: Am J Physiol Heart Circ Physiol. Volume: 305 Issue: 1 Pages: H1-H8
  • DOI: 10.1152/ajpheart.00080.2013
  • Peer Reviewed
• [Journal Article] A role for Piezo2 in EPAC1-dependent mechanical allodynia. (2013)
  • Author(s): Eijkelkamp N, et al.
  • Journal Title: Nature Commun. Volume: 4 Pages: 1682-1682
  • Peer Reviewed
• [Journal Article] Prevention of heart failure in mice by an antiviral agent that inhibits type 5 cardiac adenylyl cyclase. (2012)
  • Author(s): Iwatsubo K, et al.
  • Journal Title: Am J Physiol Heart Circ Physiol. Volume: 302
  • Peer Reviewed
• [Journal Article] Sarcalumenin plays a critical role in age-related cardiac dysfunction due to decreases in SERCA2a expression and activity. (2012)
  • Author(s): Jiao Q, et al.
  • Journal Title: Cell Calcium. Volume: 51 Issue: 1 Pages: 31-39
  • DOI: 10.1016/j.ceca.2011.10.003
  • Peer Reviewed
• [Journal Article] Inhibition of Phosphodiesterase Type 3 Dilates the Rat Ductus Arteriosus Without Inducing Intimal Thickening (2012)
  • Author(s): Ichikawa Y, et al.
  • Journal Title: Circulation Journal Volume: 76 Issue: 10 Pages: 2456-2464
  • DOI: 10.1253/circj.CJ-12-0215
  • NAID: 10030875150
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed
• [Journal Article] Mice Lacking Hypertension Candidate Gene ATP2B1 in Vascular Smooth Muscle Cells show Significant Blood Pressure Elevation. (2012)
  • Author(s): Kobayashi Y, et al.
  • Journal Title: Hypertension. Volume: 59 Issue: 4 Pages: 854-860
  • DOI: 10.1161/hypertensionaha.110.165068
  • Peer Reviewed
• [Journal Article] Acute myocardial infarction with isolated conus branch occlusion. (2012)
  • Author(s): Umemura M, et al.
  • Journal Title: J Electrocardiol. Volume: 45 Issue: 3 Pages: 285-287
  • DOI: 10.1016/j.jelectrocard.2011.11.006
  • Peer Reviewed
• [Journal Article] Effect of ascorbic acid on reactive oxygen species production in chemotherapy and hyperthermia in prostate cancer cells. (2012)
  • Author(s): Fukumura H, et al.
  • Journal Title: J. Physiol. Sci. Volume: 62 Issue: 3 Pages: 251-257
  • DOI: 10.1007/s12576-012-0204-0
  • Peer Reviewed
• [Journal Article] Pharmacological Stimulation of Type 5 Adenylyl Cyclase Stabilizes Heart Rate Under Both Microgravity and Hypergravity Induced by Parabolic Flight (2012)
  • Author(s): Bai Y, et al.
  • Journal Title: Journal of Pharmacological Sciences Volume: 119 Issue: 4 Pages: 381-389
  • DOI: 10.1254/jphs.12102FP
  • NAID: 10031071685
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Acute pulmonary embolism induced by renal obstruction with benign prostatic hyperplasia. (2012)
  • Author(s): Umemura M, et al.
  • Journal Title: Journal of Cardiology Cases. Volume: 5 Issue: 1 Pages: e39-e43
  • DOI: 10.1016/j.jccase.2011.10.004
  • Peer Reviewed
• [Journal Article] Morphological and Histological Evaluations of 3D-Layered Blood Vessel Constructs Prepared by Hierarchical Cell Manipulation. (2012)
  • Author(s): Matsusaki M, et al.
  • Journal Title: J Biomater Sci Polym Ed. Volume: 23 Issue: 1-4 Pages: 63-79
  • DOI: 10.1163/092050610x541953
  • Peer Reviewed
• [Journal Article] Inhibition of EP4 signaling attelluates aortic aneurysm formation (2012)
  • Author(s): Yokoyama U
  • Journal Title: PLoS One Volume: 7 Issue: 5 Pages: e36724-e36724
  • DOI: 10.1371/journal.pone.0036724
  • Peer Reviewed
• [Journal Article] Secretoglobin 3A2 suppresses bleomycin-induced pulmonary fibrosis by TGFbeta signaling down-regulation. (2011)
  • Author(s): Kurotani R, et al
  • Journal Title: J. Biol. Chem. Volume: 286 Issue: 22 Pages: 19682-19692
  • DOI: 10.1074/jbc.m111.239046
  • Peer Reviewed
• [Journal Article] Identification of transcription factor E3 (TFE3) as areceptor-independent activator of G α 16: Gene regulation by nuclear Ga subunit and it s activator. (2011)
  • Author(s): Sato M, et al
  • Journal Title: J Biol Chem. Volume: 286 Issue: 20 Pages: 17766-17776
  • DOI: 10.1074/jbc.m111.219816
  • Peer Reviewed
• [Journal Article] DNA microarray profiling identified a new role of growth hormone in vascular remodeling of rat ductus arteriosus. (2011)
  • Author(s): Jin M-H, et al
  • Journal Title: J Physiol Sci. Volume: 61 Issue: 3 Pages: 167-179
  • DOI: 10.1007/s12576-011-0133-3
  • Peer Reviewed
• [Journal Article] Apoptosis in Heart Failure - The Role of the .BETA.-Adrenergic Receptor-Mediated Signaling Pathway and p53-Mediated Signaling Pathway in the Apoptosis of Cardiomyocytes - (2011)
  • Author(s): Fujita T, Ishikawa Y
  • Journal Title: Circulation Journal Volume: 75 Issue: 8 Pages: 1811-1818
  • DOI: 10.1253/circj.CJ-11-0025
  • NAID: 10029127431
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed
• [Journal Article] The roles of cytochrome P450 in ischemic heart disease. (2011)
  • Author(s): Sato M, et al
  • Journal Title: Curr Drug Metab. Volume: 12 Issue: 6 Pages: 526-532
  • DOI: 10.2174/138920011795713715
  • Peer Reviewed
• [Presentation] 新生児期における浸透圧感受性TRPチャネルの役割. (2013)
  • Author(s): 横山詩子, 青木理加, 南沢享, 石川義弘
  • Organizer: 筋生理の集い
  • Place of Presentation: 東京慈恵会医科大学(東京都)
  • Year and Date: 2013-12-21
• [Presentation] Store-operated Ca2 entry (SOCE) regulates melanoma progression. (2013)
  • Author(s): Umemura M, Baljinnyam E, Lorenzo M. S. De, Feske S, Xie L, Ishikawa Y, and Iwatsubo K
  • Organizer: The 104 American Association for Cancer Research Annual meeting.
  • Place of Presentation: Washington DC, USA
• [Presentation] Application of a novel nano-magnetic anti-cancer drug to hyperthermia. (2013)
  • Author(s): Umemura M, Fukumura H, Sato I, Feng X, Izuka H, Eguchi H, Ishikawa Y. q
  • Organizer: The 104 American Association for Cancer Research Annual meeting.
  • Place of Presentation: Washington DC, USA
• [Presentation] Targeted drug delivery system and magnetic resonance imaging with intrinsic ferromagnetic nano-particle compound. (2013)
  • Author(s): Eguchi H, Hirata K, Kurotani R, Fukumura H, Singh D. J, Yam amoto M, Sato I, Umemura M, Yamamoto M, Nagashima Y, Ish ikawa Y.
  • Organizer: The 104 American Association for Cancer Research Annual meeting.
  • Place of Presentation: Washington DC, USA
• [Presentation] Thermochemotherapy with controlled drug delivery using a novel magnetic anti-cancer drug. (2013)
  • Author(s): Sato I, Masudo K, Umemura M, Feng X, Fukumura H, Eguchi H, Nakashima H, Kioi M, Tohnai I.
  • Organizer: The 104 American Association for Cancer Research Annual meeting.
  • Place of Presentation: Washington DC, USA
• [Presentation] Novel thermo-chemotherapy using a new magnetic anti-cancer drug. (2013)
  • Author(s): Sato I, Umemura M, Mitsudo K, Feng X, Baba J, Nakashima H, Kioi M, Eguchi H, Tohnai I, Is hikawa Y.
  • Organizer: The 104 American Association for Cancer Research Annual meeting.
  • Place of Presentation: Washington DC, USA
• [Presentation] Inhibitor of cardiac adenylyl cyclase prevents atrial fibrillation in mice. HD Physiology. (2013)
  • Author(s): Suita K, Cai W, Jin H, Jin M, Fujita T, Okumura S, Ishikawa Y.
  • Organizer: HD Physiology, the 2nd International Symposium.
  • Place of Presentation: 丸ノ内MYPLAZA(東京都)
• [Presentation] Mouse Overexpressing Prostaglandin E Receptor EP4 in Vascular Smooth Muscle Cells Decreased Elasticity of the Aorta. (2013)
  • Author(s): Ichikawa Y, Yokoyama U, Jin M, Ishikawa Y.
  • Organizer: The 7th TAKAO International symposium.
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京都)
• [Presentation] Prostaglandin E2-EP4 signaling promotes secretion of the lipid-relating proteoglycan buglycan in human coronary arteries during development. (2013)
  • Author(s): Kumagaya S, Yokoyama U, Sato A, Nishihara H, Inoue T, Mina misawa S, Ishikawa Y.
  • Organizer: The 7th TAKAO International symposium.
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京都)
• [Presentation] Prostaglandin E2-EP4 signaling inhibits vascular elastic fiber formation in the ductus arteriosus. (2013)
  • Author(s): Ishiwata R, Yokoyama U, Jin M, Masuda M, Asou T, Sugimoto Y, Aoki H, Nakamura T, Minam isawa S, Ishikawa Y.
  • Organizer: The 7th TAKAO International symposium.
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京都)
• [Presentation] Cardiac overexpression of Translationally Controlled Tumor Protein (TC TP) prevents the development of doxorubicin-induced heart failure. (2013)
  • Author(s): Fujita T, Cai W, Hidaka Y, Jin H, Jin M, Suita K, Ishikawa Y.
  • Organizer: European Socirty of Cardiology
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] A novel nano-magnetic particle with cytotoxic and a novel technology of designing intrinsic ferromagnetism compound. (2013)
  • Author(s): Umemura M, Eguchi H, Ishikawa Y.
  • Organizer: The 72nd Annual Meeting of the Japanese Cancer Association.
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] A conventional anti-cancer drug designed artificially with ferromagnetic, EI2573M. (2013)
  • Author(s): Iwai M, Umemura M, Sato I, N agasako A, Feng X, Hoshino Y, Inoue S, Aoki I, Eguchi H.
  • Organizer: The 72nd Annual Meeting of the Japanese Cancer Association.
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Therapeutic Effect of EP4 Antagonist on Advanced Abdominal Aortic Aneurysm. (2013)
  • Author(s): Yokoyama U, Ishiwata R, Arakawa N, Suzuki S, Masuda M, Ish ikawa Y
  • Organizer: The 86th Scientific Session, American Heart Association.
  • Place of Presentation: Dallas, USA
• [Presentation] Decreased Serum Osmolality Augments the Closure of the Ductus Arter iosus in Neonates. (2013)
  • Author(s): Yokoyama U, Aoki R, Ichikawa Y, Iwasaki S, Seki K, Nishimaki S, Yokota S, Minamisawa S, Ishikawa Y.
  • Organizer: The 86th Scientific Session, American Heart Association.
  • Place of Presentation: Dallas, USA
• [Presentation] Prostaglandin E2 Receptor EP4 Signaling in Vascular Smooth Muscle D ecreased Elasticity of the Aorta. (2013)
  • Author(s): Ichikawa Y, Yokoyarna U, Iwamoto M, Yokota S, Minamisawa S, Ichikawa Y.
  • Organizer: The 86th Scientific Session, American Heart Asso ciation.
  • Place of Presentation: Dallas, USA
• [Presentation] Anti-fibrotic Effect of Prostaglandin E2-EP4 in the Heart. (2013)
  • Author(s): Ishiwata R, Yokoyama U, Inoue S, Ichikawa Y, Ishikawa Y.
  • Organizer: The 86th Scientific Session, American Heart Asso ciation.
  • Place of Presentation: Dallas, USA
• [Presentation] 温熱治療における新規磁性抗がん剤の役割 (2013)
  • Author(s): 石川義弘
  • Organizer: 第30回日本ハイパーサーミア学会
  • Place of Presentation: 横浜シンポジア(神奈川県)
  • Invited
• [Presentation] 新規抗癌剤を用いた口腔癌に対するハイパーサーミアの開発. (2013)
  • Author(s): 佐藤 格, 光藤健司, 梅村将就, 江口晴樹, 石川義弘, 藤内 祝
  • Organizer: 第37回日本頭頸部癌学会
  • Place of Presentation: 京王プラザホテル(東京都)
• [Presentation] EP4シグナル制御による大動脈瘤の治療開発. (2013)
  • Author(s): 石渡 遼, 横山詩子, 石川義弘
  • Organizer: 新学術領域「自然炎症」+「脂質マシナリー」若手ワークショップ
  • Place of Presentation: ルネッサンスリゾート鳴門(徳島県)
• [Presentation] 出生後血清浸透圧変化からみた動脈管開存症発症リスク因子の検討. (2013)
  • Author(s): 青木理加, 横山詩子, 藤田秀次郎, 市川泰広, 岩崎志穂, 関 和男, 西巻 滋, 横田俊平, 石川義弘
  • Organizer: 第49回日本周産期新生児学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] 大動脈瘤進展におけるプロスタグランディンE-EP4の役割. (2013)
  • Author(s): 石渡 遼, 横山詩子, 石川義弘
  • Organizer: 第6回大動脈分子病態研究会
  • Place of Presentation: 久留米大学循環器病研究所(福岡県)
• [Presentation] 抗がん作用を有する新規磁性微粒子を用いたハイパーサーミアへの応用 (2013)
  • Author(s): 佐藤 格, 光藤健司, 梅村将就, 來生 知, 中島英行, 馮 先鋒, 江口晴樹, 石川義弘, 藤内 祝
  • Organizer: 第30回ハイパーサーミア学会
  • Place of Presentation: 横浜シンポジア(神奈川県)
• [Presentation] Prostaglandin Eシグナルによるlysyl oxidase分解亢進が動脈管弾性線維の形成不良を引き起こす. (2013)
  • Author(s): 南沢享, 横山詩子, 青木浩樹, 中邨智之, 石川義弘
  • Organizer: 生理研研究会―心血管膜輸送分子の構造・機能・病態の統合的研究戦略
  • Place of Presentation: 自然科学研究機構生理学研究所(愛知県)
• [Presentation] 心臓型アデニル酸シクラーゼの選択的阻害剤ビダラビンによる不整脈治療の可能性. (2013)
  • Author(s): 吹田憲治, 藤田孝之, 石川義弘
  • Organizer: 生理学研究所研究会, ―心血管膜輸送分子の構造・機能・病態の統合的研究戦略
  • Place of Presentation: 自然科学研究機構生理学研究所(愛知県)
• [Presentation] Prostaglandin E2 receptor EP4 in coronary smooth muscle cells may play a role in intimal thickening that precedes atherosclerosis. (2012)
  • Author(s): Kumagaya S, Yokoyama U, Iwai K, Nishihara H, Inoue T, Mimamisawa S, Ishikawa Y
  • Organizer: 第2回生命医科ポスター交流会
  • Place of Presentation: 東京女子医科大学・早稲田大学連携先端生命医科学研究教育施設(TWIns) (東京都)
  • Year and Date: 2012-08-10
• [Presentation] cAMPシグナルとニコチン受容体. (2012)
  • Author(s): 石川義弘
  • Organizer: 第27回喫煙科学研究財団平成23年度助成研究発表会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-07-22
• [Presentation] 酸素による動脈管リモデリング. (2012)
  • Author(s): 金美花, 横山 詩子, 石渡遼, 石川義弘
  • Organizer: 平成24年度生理学若手研究者フォーラム「生体機能へのアプローチ」
  • Place of Presentation: 順天堂大学(東京都)
  • Year and Date: 2012-06-30
• [Presentation] Phosphodiesterase Type 3 Inhibitor, Milrinone, Dilates the Rat Ductus Arteriosus without Inducing Intimal Thickening. (2012)
  • Author(s): Ichikawa y Yokoyama U, Masuda M, Asou T, Ishikawa Y
  • Organizer: The 8^<th> Congress of Asian Society for Pediatric Research.
  • Place of Presentation: Seoul, Korea
• [Presentation] Rat SMCs within 3D Cellular Mutilayers Exhibit Contractile Phenotypes and Layered elastic fibe. (2012)
  • Author(s): Ishiwata R, Yokoyama U, Kadowaki K, Matsusaki M, Akashi M, Ishikawa Y, Minamisawa S
  • Organizer: The 8^<th> Congress of Asian Society for Pediatric Research.
  • Place of Presentation: Seoul, Korea
• [Presentation] Prostaglandin E2 receptor EP4 expressed in coronary smooth muscle cells may play a role in intimal thickening that precedes atherosclerosis. (2012)
  • Author(s): Kumagaya S, Yokoyama U, Iwai K, Nishihara H, Inoue T, Mimamisawa S, Ishikawa Y
  • Organizer: The 8^<th> Congress of Asian Society for Pediatric Research.
  • Place of Presentation: Seoul, Korea
• [Presentation] Plasma Hypoosmolarity after Birth Promotes Closure of the Ductus Arteriosus. (2012)
  • Author(s): Aoki R, Yokoyama U, Ichikawa Y, Minamisawa S, Ishikawa Y
  • Organizer: The 86^<th> American heart association basic cardio vascular science 2012 Scientific session.
  • Place of Presentation: New Orleans, USA
• [Presentation] Hyperthermnia and nanotechnology/nanomedicine Magnetic anti-cancer compound in hyperthermic therapy. (2012)
  • Author(s): Ishikawa Y
  • Organizer: ICHO&JCTM 2012.
  • Place of Presentation: 国立京都国際会議場(京都府)(招待講演)
• [Presentation] Development of simultaneous hyperthermo-chemotherapy using a drug. (2012)
  • Author(s): Sato l, Mitsudo K, Baba J, Iida M, Nakashima H, Koizumi T, Kioi M, Eguchi H, Ishikawa Y, Tohnai I
  • Organizer: ICHO&JCTM 2012.
  • Place of Presentation: 国立京都国際会議場(京都府)
• [Presentation] A new cancer treatment strategy with magnetic anti-cancer compound with hyperthermia. (2012)
  • Author(s): Feng X, Fukumura H, Sato I, Eguchi H, Ishikawa Y
  • Organizer: The 11th International Congress of Hyperthermic Oncology, The 29th Japanese Congress of Ther mal Medicine.
  • Place of Presentation: 国立京都国際会議場(京都府)
• [Presentation] Inhibition of Phosphodiesterase Type 3 Dilates the Rat Ductus Arteriosus without Inducing Intimal Thickening. (2012)
  • Author(s): Ichikawa y Yokoyama U, Masuda M, Asou T, Ishikawa Y
  • Organizer: The 86^<th> American Heart Association Scientific Session.
  • Place of Presentation: Los Angeles, USA
• [Presentation] Oxygenation-induced postnatal remodeling of the ductus arteriosus. (2012)
  • Author(s): Jin MH, Yokoyama U, Ishiwata R, Minamisawa S, Ishikawa Y
  • Organizer: The 86^<th> American Heart Association Scientific Session.
  • Place of Presentation: Los Angeles, USA
• [Presentation] Disruption of Epacl decreases phosphorylation of phospholamban and protects the heart against stress. (2012)
  • Author(s): Okumura S, Jin M, Ohnuki Y, Namekata l, Kurotani R, Fujita T, Jin HL, Cai W, Bai Y, Suita K, Tsunematsu T, Tanaka H, Ishikawa Y
  • Organizer: The 86^<th> American Heart Association Scientific Session.
  • Place of Presentation: Los Angeles, USA
• [Presentation] 大動脈瘤形成におけるプロスタグランディンE受容体EP4の役割の検討. (2012)
  • Author(s): 片山雄三、横山詩子、岩本眞理、根本寛子、笠間啓一郎、鈴木伸一、磯松幸尚、内田敬二、井元清隆、石川義弘、益田宗孝
  • Organizer: 第42回日本心臓血管外科学会学術集会
  • Place of Presentation: 秋田大学(秋田県)
• [Presentation] 物理的作用を活用した高機能ドラッグデリバリーシステム、磁性抗癌剤を用いた癌治療. (2012)
  • Author(s): 石川義弘
  • Organizer: 第51回日本生体医工学大会
  • Place of Presentation: 福岡国際会議場(福岡県)
• [Presentation] 出生後における肺の弾性線維形成の検討. (2012)
  • Author(s): 市川泰広, 横山詩子, 岩本眞理, 南沢享, 石川義弘
  • Organizer: 第48回日本小児循環器学会
  • Place of Presentation: 国立京都国際会館(京都府)
• [Presentation] ラット動脈管リモデリングにおけるprostaglandin E2- Nuclear Factor kappa B経路の活性化. (2012)
  • Author(s): 梶村いちげ, 横山詩子, 石川義弘, 南沢享
  • Organizer: 第48回日本小児循環器学会
  • Place of Presentation: 国立京都国際会館(京都府)
• [Presentation] アミノ酸輸液の組成の違いが動脈管閉鎖に及ぼす作用の検討. (2012)
  • Author(s): 藤田秀次郎, 横山詩子, 青木理加, 小郷寛史, 岩崎志穂, 西巻滋, 関和男, 石川義弘
  • Organizer: 第48回日本周産期・新生児医学会学術集会
  • Place of Presentation: 大宮ソニックシティ(埼玉県)
• [Presentation] CaとcAMPシグナル. (2012)
  • Author(s): 石川義弘
  • Organizer: 文部科学省平成24年度新学術領域研究「統合的多階層生体機能学領域の確立とその応用」第5回全体会議
  • Place of Presentation: 福岡大学(福岡県)
• [Presentation] Store-operated Ca2+ entry regulates proliferation and cell cycle in melanoma. (2012)
  • Author(s): Umemura M, Erdne B, Lai-HuaX, Marianas D.L, Ishikawa Y, Umemura S, Iwatsubo K
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌, 他 (北海道)
• [Presentation] 心臓型アデニル酸シクラーゼ選択的抑制薬を用いた新しい心不全治療 (2011)
  • Author(s): 奥村敏, 川又史佳, 金慧玲, 蔡文倩, 金美花, 日高祐子,吹田憲治, 岩坪耕策, 石川義弘
  • Organizer: 第6回 高血圧と冠動脈疾患研究会.
  • Place of Presentation: 大手町サンケイプラザ(東京都)
  • Year and Date: 2011-12-23
• [Presentation] PDE3阻害薬の動脈管拡張薬としての検討. (2011)
  • Author(s): 市川泰広, 横山詩子, 石川義弘
  • Organizer: 厚生労働省科学研究費成果発表会
  • Place of Presentation: 横浜市立大学(神奈川県)
  • Year and Date: 2011-09-18
• [Presentation] Three dimensional cellular multi-layer technology utilized evaluation of elastic fiber formation and phenotype of rat vascular smooth muscle cells. (2011)
  • Author(s): Ishiwata R, Yokoyama U, Kado waki K, Matsusaki M, Akashi M, Ishikawa Y, Minamisawa S
  • Organizer: The 7th Japan-China-Korea Pediatric Heat Forum
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2011-07-08
• [Presentation] Epac activation protects heart from interleukin-6-induced cardiac dysfunction by inhibiting STAT/iNOS signaling. (2011)
  • Author(s): Okumura S, Jin M, Kawamata F, Jin H-L, Cai W, Bai Y, Suit a K, Hidaka Y, Tsunematsu T, Ishikawa Y
  • Organizer: The 85^<th> American Heart Association.
  • Place of Presentation: Orlando, USA
• [Presentation] Cardiac protection from hypoxic inury by a novel synthetic peptide targeting the activator of G-protein signaling 8-mediated GBy signal. (2011)
  • Author(s): Motohiko Sato, Masahiro Hiraoka, Yukiko Yamane, xianfeng Feng, Yoshihiro Ishikawa
  • Organizer: The 85th American Heart Association.
  • Place of Presentation: Orlando, USA
• [Presentation] Epacl Deficiency Inhibit Neointima Formation after Vascular Injury in vivo. (2011)
  • Author(s): Kato Y, Yokoyama U, Okumura S, Minamisawa S, Sata M, Miyajima E, Ishikawa Y
  • Organizer: The 85th American Heart Association.
  • Place of Presentation: Orlando, USA
• [Presentation] 動脈管収縮における出生後の血清浸透圧低下の役割. (2011)
  • Author(s): 青木理加, 横山詩子, 岩崎志穂, 関和男, 西巻滋, 横田俊平, 石川義弘
  • Organizer: 第56回日本未熟児新生児学会学術集会
  • Place of Presentation: 東京国際フォーラム(東京都)
• [Presentation] 動脈管閉鎖における低浸透圧センサーTransient Receptor Potential Melastatin 3 (TRPM3)チャネルの役割.心血管イオンチャネル・トランスポーク一研究の新展開 基礎研究と臨床研究の融合. (2011)
  • Author(s): 青木理加, 横山詩子, 市川泰広, 南沢享, 石川義弘
  • Organizer: 生理学研究所研究会
  • Place of Presentation: 生理学研究所(愛知県)
• [Presentation] 酸素による動脈管リモデリング (2011)
  • Author(s): 金美花, 横山詩子, 石渡遼, 南沢享, 石川義弘
  • Organizer: 第10回心臓血管発生研究会
  • Place of Presentation: 磐梯熱海温泉ホテル華の湯(福島県)
• [Presentation] Epaclは細胞極性を促進し内膜肥厚形成に関与する. (2011)
  • Author(s): 加藤優子, 横山詩子, 奥村敏, 南沢享, 佐田政隆, 宮島栄治, 石川義弘
  • Organizer: Cardiovascular Conference II
  • Place of Presentation: キロロホテルピアノ(北海道)
• [Presentation] Prostaglandin EP4 Signaling Negatively Regulates Vascular Elastic Fiber Assembly. (2011)
  • Author(s): Yokoyama U, Kato Y, Suzuki S, Masuda M, Asou T, Aoki H, Sugimoto Y, Nakamura T, Minamisawa S, Ishikawa Y
  • Organizer: The 2nd Molecular Cardiovascular Conference II
  • Place of Presentation: キロロホテルピアノ(北海道)
• [Presentation] Epaclは血管障害時の内膜肥厚形成を促進する. (2011)
  • Author(s): 加藤優子, 横山詩子, 奥村敏, 南沢享, 佐田政隆, 宮島栄治, 石川義弘
  • Organizer: 第53回日本平滑筋学会
  • Place of Presentation: ゆうぽうと(東京都)
• [Presentation] Disruption of Epacl Protects Heart from Catecholamine-mediated Car diac Fibrosis. (2011)
  • Author(s): Okumura S, Cai W, Jin M, Bai Y, Tsunematsu T, HidakaY, Kurotani R, Ishikawa Y
  • Organizer: 第75回日本循環器学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] 出生後の血清浸透圧低下は動脈管収縮を促進させる. (2011)
  • Author(s): 市川泰広, 青木理加, 横山詩子, 岩本眞理, 南沢享, 石川義弘
  • Organizer: 第47回日本小児循環器学会総会・学術集会
  • Place of Presentation: 福岡国際会議場(福岡県)
• [Book] 遺伝子医学MOOK24 最新生理活性脂質研究-実験手法、基礎的知識とその応用-「大動脈瘤の進展とPGE2」 (2013)
  • Author(s): 横山詩子、金美花、石川義弘
  • Total Pages: 312
  • Publisher: メディカルドゥ社
• [Book] TPP問題の核心 公平な医療を受けるため「国民皆保険」を守れ.まだ知らされていない壊国TPP-主権侵害の招待を暴く- (2012)
  • Author(s): 石川義弘
  • Publisher: 創森社
• [Book] ボロン・ブールペープ 生理学2005. (2011)
  • Author(s): 泉井亮, 監訳河南洋, 久保川学, 訳青木史暁, 赤池紀生, 石川義弘, 石山延吉, 上田陽一, 上野伸哉, 大野忠雄, 河原克雅, 河南洋, 北村竜一, 久保川学, 桑木共之,小島至, 佐々木和彦, 高瀬堅吉, 槌田成紀, 照井直人, 中村晃, 花森隆充, 福田康一郎, 藤原広明, 船橋利也, 山崎将生, 山田聡子, 山本頼綱, 泉井亮
  • Total Pages: 1363
  • Publisher: 西村書店
• [Remarks] 横浜市立大学大学院医学研究科 循環制御医学 ホームページ
  • URL: http://www-user.yokohama-cu.ac.jp/~seiril/
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~seiri/index.html
• [Remarks]
• [Patent(Industrial Property Rights)] 三次元組織体およびその製造方法 (2013)
  • Inventor(s): 横山詩子, 石川義弘, 明石 満, 松崎典弥
  • Industrial Property Rights Holder: 公立大学法人横浜市立大学国立大学法人大阪大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-10-10