| Project/Area Number |
12307053
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
KIRINO Yutaka The University of Tokyo, Graduate School of Pharmaceutical Sciences, Professor, 大学院・薬学系研究科, 教授 (10012668)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Tetsuya Institute for Phisical and Chemical Researches (RIKEN), Brain Science Institute, Research Staff, 脳科学総合研究センター, 研究員
WATANABE Satoshi The University of Tokyo, Graduate School of Pharmaceutical Sciences, Research Associate, 大学院・薬学系研究科, 助手 (80302610)
KAWAHARA Shigenori The University of Tokyo, Graduate School of Pharmaceutical Sciences, Associate Professor, 大学院・薬学系研究科, 助手 (10204752)
MATSUO Ryota The University of Tokyo, Graduate School of Pharmaceutical Sciences, Research Associate, 大学院・薬学系研究科, 助手 (40334338)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥41,100,000 (Direct Cost: ¥36,300,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2002: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2001: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2000: ¥20,300,000 (Direct Cost: ¥20,300,000)
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| Keywords | slug / associative learning / procerebrum / nonbursting neuron / inhibitory postsynaptic potential / in vitro learning / burst dischanges / nystatin perforated patch recording / 遺伝子導入 / 長期記憶 / 遺伝子発現 / differential display / RNAi / 神経修飾物質 / タンパク合成 |
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| Research Abstract |
Slugs perform odor-taste associative learning, and the present work was aimed at clarifying the mechanisms of odor learning using physiological and molecular biological methods. A high frequency stimulation of the lip nerve, which is an in vitro analog of the unconditioned stimulus, caused an increased frequency in the local Meld potential oscillation in the procerebrum (PC), as well as excitation of the bursting PC neurons and inhibition of the nonbursting PC neurons. As the nonbursting neurons are thought to be the principal neurons of the PC and may mediate output of information from the PC to other brain regions, these results suggest that output of information from the PC is decreased during conditioning. A high frequency stimulation of the tentacle nerve resulted in a potentiation of the evoked potential in the PC, which lasted for more than 2 h. Such plastic changes in the efficacy of synaptic transmission in the PC may be involved in odor learning.
Injection of the protein synthesis inhibitors anisomycin or cycloheximide impaired memory retention after 2-7 days alter conditioning, indicating that protein synthesis is necessary for the retention of long-term memory. We further explored genes induced by the odor-taste associative conditioning using the PCR-differential display method in the brains from paired and unpaired conditioned animals. Using 100 pairs of random primers, 12 bands showed a reproducible induction pattern, and partial sequences of 8 genes were cloned.
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