| Project/Area Number |
12359001
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
広領域
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| Research Institution | Tohoku University |
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Principal Investigator |
SHIMOSEGAWA Tooru tohoku university, graduate school of medicine, professor, 大学院・医学系研究科, 教授 (90226275)
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| Co-Investigator(Kenkyū-buntansha) |
ASAKURA Tohru Tohoku University, graduate school of medicine, research associate, 大学院・医学系研究科, 助手 (20272023)
TASHIRO Hideo Riken, probing technology laboratory, chief Scientist, 計測工学研究室, 主任研究員 (90124370)
SATO Hidetoshi Riken, probing technology laboratory, researcher, 計測工学研究室, 研究員 (10300873)
IMATANI Akira Tohoku University, graduate school of information science, research associate, 大学院・情報科学研究科, 助手 (30333876)
AIZAWA Katuo Tokyo medical university, physiology 2^<nd>, professor, 第二生理学, 教授 (40074645)
佐藤 賢一 東北大学, 医学部・附属病院, 助手 (10282055)
池田 照樹 日本分光(株), 第一技術部, 次長
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| Project Period (FY) |
2000 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥41,170,000 (Direct Cost: ¥37,600,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2003: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2002: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2001: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2000: ¥25,700,000 (Direct Cost: ¥25,700,000)
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| Keywords | photodynamic therapy / mono-l-aspartyl chlorin e6(Npe 6) / raman specrtoscopy / Ti:sapphire laser / optical biopsy / fiber optic probe / ラマン散乱 / 波長可変レーザー / 生体分光 / 光ファイバー / 消化器腫瘍 |
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| Research Abstract |
To clarify the usefulness of the second-generation photosensitizer, mono-L-aspartyl chlorin e6(NPe6), we examined the possibility of photodynamic diagnosis and photodynamic therapy for intestinal tumors in a mouse model. (1) The intestinal tumor sites were irradiated with a 664-nm diode laser constant intervals after the administration of photosensitizers, respectively. (2) The tumors were excised and processed for the frozen section to observe the fluorescence by a microscope. We observed the fluorescent image and calculated that the mean fluorescence intensity was significantly higher in the tumors than the normal mucosa during 6 hours (P<0.001). The fluorescence of NPe6 was chiefly accumulated in the intestinal tumors as red fluorescence on the fluorescent microphotographic image. We conclude that NPe6 may be a valuable photosensitizer for the photodynamic diagnosis and photodynamic therapy of intestinal tumors.
Our major goal is to develop advanced analytical techniques for biomedical researches based on the Raman spectroscopic applications of an electronically tuned Ti:sapphire laser and the developments of advanced spectroscopic tools.
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