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Fiber-optic approaches for diagnosis of gastrointestinal disease

Research Project

Project/Area Number 12359001
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 広領域
Research InstitutionTohoku University

Principal Investigator

SHIMOSEGAWA Tooru  tohoku university, graduate school of medicine, professor, 大学院・医学系研究科, 教授 (90226275)

Co-Investigator(Kenkyū-buntansha) ASAKURA Tohru  Tohoku University, graduate school of medicine, research associate, 大学院・医学系研究科, 助手 (20272023)
TASHIRO Hideo  Riken, probing technology laboratory, chief Scientist, 計測工学研究室, 主任研究員 (90124370)
SATO Hidetoshi  Riken, probing technology laboratory, researcher, 計測工学研究室, 研究員 (10300873)
IMATANI Akira  Tohoku University, graduate school of information science, research associate, 大学院・情報科学研究科, 助手 (30333876)
AIZAWA Katuo  Tokyo medical university, physiology 2^<nd>, professor, 第二生理学, 教授 (40074645)
佐藤 賢一  東北大学, 医学部・附属病院, 助手 (10282055)
池田 照樹  日本分光(株), 第一技術部, 次長
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥41,170,000 (Direct Cost: ¥37,600,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2003: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2002: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2001: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2000: ¥25,700,000 (Direct Cost: ¥25,700,000)
Keywordsphotodynamic therapy / mono-l-aspartyl chlorin e6(Npe 6) / raman specrtoscopy / Ti:sapphire laser / optical biopsy / fiber optic probe / ラマン散乱 / 波長可変レーザー / 生体分光 / 光ファイバー / 消化器腫瘍
Research Abstract

To clarify the usefulness of the second-generation photosensitizer, mono-L-aspartyl chlorin e6(NPe6), we examined the possibility of photodynamic diagnosis and photodynamic therapy for intestinal tumors in a mouse model. (1) The intestinal tumor sites were irradiated with a 664-nm diode laser constant intervals after the administration of photosensitizers, respectively. (2) The tumors were excised and processed for the frozen section to observe the fluorescence by a microscope. We observed the fluorescent image and calculated that the mean fluorescence intensity was significantly higher in the tumors than the normal mucosa during 6 hours (P<0.001). The fluorescence of NPe6 was chiefly accumulated in the intestinal tumors as red fluorescence on the fluorescent microphotographic image. We conclude that NPe6 may be a valuable photosensitizer for the photodynamic diagnosis and photodynamic therapy of intestinal tumors.
Our major goal is to develop advanced analytical techniques for biomedical researches based on the Raman spectroscopic applications of an electronically tuned Ti:sapphire laser and the developments of advanced spectroscopic tools.

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (16 results)

All 2004 2003 2002 2001 Other

All Journal Article (11 results) Publications (5 results)

  • [Journal Article] Qualitative analysis of trace Raman bands of non-resonant species in near-infrared excited pre-resonance Raman spectra of hemoglobin by partial least square regression-Raman excitation profile method2004

    • Author(s)
      Sato H, Wada S, et al.
    • Journal Title

      Vibrational Spectroscopy 34

      Pages: 149-156

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Qualitative analysis of trace Raman bands of non-resonant species in near-infrared excited pre-resonance Raman spectra of hemoglobin by partial least square regression-Raman excitation profile method2004

    • Author(s)
      H.Sato, S.Wada, Y.Ozaki, H.Tashiro
    • Journal Title

      Vibrational Spectrosc 34

      Pages: 149-156

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 動脈硬化症診断のための分光分析技術2004

    • Author(s)
      佐藤英俊, 小町裕一, 朝倉徹, 下瀬川徹, 會沢勝夫, 田代英夫
    • Journal Title

      レーザー医学会誌 25

      Pages: 93-100

    • NAID

      10013331308

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Phetodynamic therapy of intestinal tumors with mono-L-Aspartyl chlorin e6 (Npe6): a basic study2003

    • Author(s)
      Kikuchi T, Asakura T, et al.
    • Journal Title

      Anticancer Reserch 23

      Pages: 4897-4900

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Electronically tunable-laser light sources for NIR spectroscopy.2003

    • Author(s)
      Sato H, Saito N, et al.
    • Journal Title

      J.Near Infrared Spectroscopy 11

      Pages: 295-308

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Electronically tunable-laser light sources for NIR spectroscopy.2003

    • Author(s)
      H.Sato, N.Saito, K.Akagawa, S.Wada, H.Tashiro
    • Journal Title

      J Near Infrared Spectrosc 11

      Pages: 295-308

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Photodynamic therapy of intestinal tumors with mono-l-aspartyl chlorin e6(Npe6) : a basic study2003

    • Author(s)
      T.Kikuchi, T.Asakura, H.Aihara, M.Shiraki, S.Takagi, Y.Kinouchi, K.Aizawa, T.Shimosegawa.
    • Journal Title

      Anticancer Res 23

      Pages: 4897-4900

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Fluorescence background less Ti : sapphire laser using acoust-optical tunable filter for Raman spectroscopic measurements.2002

    • Author(s)
      Sato H, Wada S, et al.
    • Journal Title

      Appl.Spectroscopy 56

      Pages: 1303-1307

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Fluorescence background less Ti:sapphire laser using acoust-optical tunable filter for Raman spectroscopic measurements2002

    • Author(s)
      H.Sato, S.Wada, H.Tashiro
    • Journal Title

      Appl Spectrosc 56

      Pages: 1303-1307

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Excitation wavelength-dependent changes in Raman spectra of whole blood and hemoglobin : comparison of the spectra with 514.5-. 720-. and 1064-nm excitation2001

    • Author(s)
      Sato H, chiba H, et al.
    • Journal Title

      J Biomed Opt 6

      Pages: 366-370

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Excitation wavelength-dependent changes in Raman spectra of whole blood and hemoglobin : comparison of the spectra with 514.5-, 720-, and 1064-nm excitation.2001

    • Author(s)
      H.Sato, H.Chiba, H.Tashiro, Y.Ozaki
    • Journal Title

      J Biomed Opt 6

      Pages: 366-370

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuchi T, Asakura T, et al.: "Photodynamic therapy of intestinal tumors with mono-L-Aspartyl chlorin e6 (Npe6): a basic study"Anticancer Reserch. 23. 4897-4900 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sato H, Wada S, et al.: "Qualitative analysis of trace Raman bands of non-resonant species in near-infrared excited pre-resonance"Vibrational Spectroscopy. 34. 149-156 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sato H, Saito K, et al.: "Electronically tunable-laser light sources for NIR spectroscopy"J.Near Infrared Spectroscopy. 11. 295-308 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hidetoshi Sato: "Raman mapping study of compatibilized and uncompatibilized polymer blends of nylon 12 and polyethylene"Appl. Spectrosco.. 56. 1038-1043 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hidetoshi Sato: "Fluorescence background less Ti : sapphire laser using acoust-optical tunable filter for Raman spectroscopic measurements"Appl. Spectrosco.. (in press). (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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