| Project/Area Number |
12440164
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TERAZIMA Masahide Graduate School of Science, Kyoto University, Professor, 大学院・理学研究科, 教授 (00188674)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 2001: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2000: ¥11,500,000 (Direct Cost: ¥11,500,000)
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| Keywords | transient grating / volume change / enthalpy change / diffusion coefficient / photoreaction / protein / folding / 拡散 / 無輻射遷移 / 励起状態 / 溶液 |
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| Research Abstract |
New photo-trigger methods were developed to study the protein dynamics and the energy changes during the chemical reactions of the proteins.
1. Protein dynamic of carboxymyoglobin was studied using the photo-dissociation reaction of the ligand. The dynamics and the energy change were revealed from nanosecond to second time range.
2. A pH jump method was developed to detect the protein structural change induced by the pH jump. The mechanism of the chemical reaction was studied.
3. Dynamics of apo-myogobin induced by the pH jump reaction was revealed.
4. Protein dynamics of cytochrome c was induced by photo-reduction method with a nanosecond pulsed laser. Thestructural change of cytochrome c after the photo-reduction was detected in terms of the volume change and the diffusion coefficient.
5. The photo-isomerization reaction of Photoactive Yellow Protein was used to initiate the cyclic reaction. A large temperature dependent volume change and change of the diffusion coefficient were discovered for the first time. The role of amino acids was revealed using the mutation technique.
6. A new reaction intermediate of octopus rhodopsin was discovered and the energy scheme of the reaction was determined.
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