| Project/Area Number |
12440242
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
系統・分類
|
|---|
| Research Institution | Okazaki National Research Institutes |
|---|
Principal Investigator |
HASEBE Misuyasu Okazaki National Research Institutes National Institutes for Basic Biology, Professor, 基礎生物学研究所, 教授 (40237996)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUJITA Tomomichi Okazaki National Research Institutes National Institutes for Basic Biology,Research Associate, 基礎生物学研究所, 助手 (50322631)
TSUKAYA Hirokazu Okazaki National Research Institutes,Center for Integative Bioscience,Associate Professor, 統合バイオサイエンスセンター, 助教授 (90260512)
MURATA Takashi Okazaki National Research Institutes National Institutes for Basic Biology,Associate Professor, 基礎生物学研究所, 助教授 (00242024)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2001: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2000: ¥7,300,000 (Direct Cost: ¥7,300,000)
|
|---|
| Keywords | KNOX / Physcomitrella patens / sporophyte / gametophyt / generation / auxin / polar transport / shoot / 平行進化 / ホメオボックス / HD-Zip |
|---|
| Research Abstract |
1. Class 1 KNOX gene of Physcomitrella patens (PpKNOX 1) was cloned and each GUS and GFP reporter gene was inserted into either 3 and 5 terminal of PpKNOX genomic region using gene targeting technique. The fusion protein was expressed only in the sporophyte generation but not in the gametophyte generation in which stems and leaves develop. This result indicates that molecular mechanisms of stems and leaves between angiosperms and Physcomitrella are different. The ectopic expression of PpKNOX1 gene in Arabidopsis caused the serrated leaves similar to the transformants expressing Arabidopsis class 1 KNOX gene, suggesting that class 1 KNOX function in angiosperms had already established in the common ancestor of angiosperms and Physcomitrella that did not yet have leaves and stems in the sporophyte generation. 2. Polar transport of auxin between gametophores and sporophytes of P. patens, Polytrichum commune, and Bartramia pomiformis was compared. We measured polar auxin transport by a feeding experiment of radiolabelied auxin in either end of the cutting segment of the shoot of mosses, and found that there was little difference of basipetal auxin transport from acropetal transport in gametophores, but obvious polar transport from apical to basal transport was observed in sporophytes. This result also support the hypothesis that leaves and stems of the gametophyte generation in mosses are differently regulated from those of the sporophyte generation in angiosperms.
|
