| Project/Area Number |
12450343
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
工業物理化学
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
WATANABE Tadashi The University of Tokyo, Institute of Industrial Science, Professor, 生産技術研究所, 教授 (70092385)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Shoichiro The University of Tokyo, Institute of Industrial Science, Research Associate, 生産技術研究所, 助手 (40111570)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2000: ¥9,100,000 (Direct Cost: ¥9,100,000)
|
|---|
| Keywords | photosynthesis / chlorophyll / reaction center / HPLC / spectrophotometry / supramolecule / protein / complex / 光化学系I / クロロフィルa' / 色素 / 二次電子受容体 / キノン / クロロフィルa′ |
|---|
| Research Abstract |
The present research has been conducted to draw a conclusion as to the actual number and the in vito functioning site of chloro-phyll a' (Chl a') which the researchers had detected in the vicinity of the reaction center P700 of photosystem I for a variety of oxygenic photo-synthetic organisms. After establishing HPLC conditions by which most of the chlorophylls, carotenoids/ xanthophylls and quinines can be determined in a relatively short time, we analyzed the pigment compositions of higher plants, a thermophilic cyanobacterium, regular cyanbacteria and green alga, the stoichiometry Chl a' /P700 = 1 was concluded.
This, together with an X-ray crystallographic result on the system I reaction center of the thermophilic cyanobacterium, reported in 2001 by Jordan et al., finally has led to a conclusion that Chl a' is an essential component of P700 itself, forming a heterodimer with the normal Chl a molecule.
In addition to these, the reaction center molecular assemblies of a green sulfur bacterium and of a marine prokaryote have been elucidated for the first time.
|
