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Molecular Diagnosis of Mycoses

Research Project

Project/Area Number 12460141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionNihon University

Principal Investigator

HASEGAWA Atsuhiko  College of Bioresourse Sciences, Nihon University, Professor, 生物資源科学部, 教授 (90011923)

Co-Investigator(Kenkyū-buntansha) KANO Rui  College of Bioresourse Sciences, Nihon University, Assistant, 生物資源科学部, 助手 (00318388)
MORITOMO Tadaaki  College of Bioresourse Sciences, Nihon University, Associate Professor, 生物資源科学部, 助教授 (20239677)
WATARI Toshihiro  College of Bioresourse Sciences, Nihon University, Associate Professor, 生物資源科学部, 助教授 (50220950)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥7,000,000 (Direct Cost: ¥7,000,000)
KeywordsChitin synthase I gene / data base / rapid diagnosis / mycoses / molecular diagnosis / chitin synthase遺伝子
Research Abstract

The DNA sequences of chitin synthase (CHS) genes in dermatophytes were elucidated, and deposited on the Gene Bank, developing the data base. CHS genes in clinical isolates of dermatophytes were also analyzed and a rapid molecular identification was performed confirming the homology between the DNA sequences of clinical isolates an4 those on the data base. Therefore, the rapid molecular method was established for the identification of dermatophytes.
Furthermore, from biopsy specimens from the lesions of fungal infection, as well as clinical samples of blood, and excretions, CHS genes, ribosomal DNA, intertranscribed space (ITS) range DNA were cloned. and sequenced ,to compare the DNA sequences reported on data base. By these molecular analysis, etiologic fungi including dermatophytes were identified.
Simultaneously, the isolates from these clinical samples were also examined molecularly proving that they were consisting to the results obtained directly from these clinical specimens. The results were also confirmed by classical identification methods.
As mentioned above, the molecular method we developed was evaluated to be more rapid and easier with good quality in sensitivity and in specificity for the diagnosis of fungal infection rather than the previous method used in general. We have been carrying out this new molecular method to identify the clinical isolates emerging and to diagnose the animal cases, re-emerging fungal pathogens in animals, respectively and the diversity of animal mycoses. All obtained this research were already published on the international journals.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kano R, Konishi K, Nakata K, Komatsu S, Nomura M, Okuzumi K, Hasegawa A: "Isolation of Candida krusei from a case of bovine bronchopneumonia in a one-year-old heifer"Vet.Rec.. 148. 636 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aizawa T, Kano R, Nakamura Y, Watanabe S, Hasegawa A: "The genetic diversity of clinical isolates of M.pachydermatis from dogs and cat"Med.Myocol.. 39. 329-334 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kano R, Sakamoto Y, Hanahachi A, Kamata H, Fukuda Y, Fujiwara, K, Hasegawa A: "Molecular identification of Candida parapsilosis from crop mucosa in a cockatiel"J.Vet.Diag.Invest. 13. 437-439 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mochizuki T, Kawasaki M, Ishizaki H, Kano R, Hasegawa A, Tosaki H, Fujihiro M.: "Molecular epidemiology of Arthroderma benhamiae, an emerging pathogen of dermatophytoses in Japan, by polymorphisms of the non-transcribed spacer region of the ribosomal DNA"J.Dermatol.Sci. 27. 4-20 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kano R, Fujino Y, Takamoto N, Tsujimoto H, Hasegawa A: "PCR detection of the Cryptococcus neoformans CAP59 gene from a biopsy specimen from a case of feline Cryptococcosis"J.Vet.Diag.Invest.. 13. 439-442 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito K, Kano R, Nakamura Y, Watanabe S, Hasegawa A: "Arthroderma benhamiae infection in a rabbit"J.Vet.Med.Sci.. 63. 929-931 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K ano R, Konishi K, Nakata K,Sano K, Komatsu S, Nomura M, Okuzumi K and Hasegawa A.: "Isolation of Candida krusei from a case of bovine bronchopneumonia in a one-year-old heifer"VET. Rec.. 148. 636 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aizawa T, Kano R, Nakamura Y, Watanabe S. and Hasegawa A: "The genetic diversitiy of clinical Isolates of M..pachydermatis"Med. Mycol.. 39. 329-334 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kano R, Sakamoto Y, Hamahati A, Kamata H, Fukuda Y, Fujiwara K. and Hasegawa A.: "Molecular identification of Candida parapsilosis from crop mucosa in a cockatiel"J. Vet. Diag. Invest.. 13. 437-439 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mochizuki T, Kawasaki M, Ishizaki H, Kano R, Hasegawa A, tosaki H, Fujihiro M.: "Molecular epidemiology of Arthroderma benhamiaae, an emerging pathogen of dermatophytoses In Japan, by polymorphisms of the non-transcribed spacer region of the ribosomal DNA"J. Dematol. Sci.. 27. 4-20 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kano R, Fujino Y, Takamoto N, Tsujimoto H. and Hasegawa A.: "PCR detection of the Cryptococcus neoformans CAP59 gene from a biopsy specimen from A case of feline Cryptococcosis"J. Vet. Diag. Invest.. 13. 439-442 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito K, Kano R, Nakamura Y, Watanabe S. and Hasegawa A.: "Arthroderma benhamiae infection in a rabbit"J. Vet. Med. Sci.. 63. 929-931 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kano R, Konishi K, Nakata K, Sano K, Komatsu S, Nomura M, Okuzumi K, Hasegawa A: "Isolation of Candida krusei from a case of bovine bronchopneumonia in a one-year-old heifer"Vet.Rec.. 148. 636 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Aizawa T, Kano R, Nakamura Y, Watanabe S, Hasegawa A: "The genetic diversity of clinical isolates of M.pachydermatis from dogs and cats"Med.Myocol.. 39. 329-334 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kano R, Sakamoto Y, Hanahachi A, Kamata H, Fukuda Y, Fujiwara, K, Hasegawa A: "Molecular identification of Candida parapsilosis from crop mucosa in a cockatiel"J.Vet.Diag.Invest. 13. 437-439 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mochizuki T, Kawasaki M, Ishizaki H, Kano R, Hasegawa A, Tosaki H, Fujihiro M.: "Molecular epidemiology of Arthroderma benhamiae,an emerging pathogen of dematophytoses in Japan,by polymorphisms of the non-transcribed spacer region of the ribosomal DNA"J.Dermatol.Sci. 27. 4-20 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kano R, Fujino Y, Takamoto N, Tsujimoto H, Hasegawa A: "PCR detection of the Cryptococcus neoformans CAP59 gene from a biopsy specimen from a case of feline Cryptococcosis"J.Vet.Diag.Invest.. 13. 439-442 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saito K, Kano R, Nakamura Y, Watanabe S, Hasegawa A: "Arthroderma benhamiae infection in a rabbit"J.Vet.Med.Sci.. 63. 929-931 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kano R,Nakamura Y,Watanabe S and Hasegawa A: "Detection of Microsporum canis in the skin scrapings of dogs with dermatophytosis based on sequences of the chitin synthase I gene"Micobiol.Immunol.. 44. 605-607 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kano R,Makimura K,Kushida T,Nomura M,Yamaguchi H and Atsuhiko Hasegawa: "First isolation of Stephanoascus ciferrii from a cat"Micobiol.Immunol. 44. 711-713 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kano R,Kusuda M,Nakamura Y,Watanabe S,Tsujimoto H and Hasegawa A: "First isolation of Exophiala dermatitidis from a dog"Vet.Microbiol.. 76. 201-205 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kano R,Aihara S,Nakamura Y,Watanabe S and Hasegawa A: "Chitin synthase 1 (Chsl) gene sequences of Microsporum equinum and Trichophyton equinum"Vet.Microbiol. 78. 85-90 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kano R,Yasuda K,Nakamura Y and Hasegawa A: "Microsporum gypseum isolated from a feline case of dermatophytosis"Mycoses. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kano R,Nakamura Y,Watanabe S,Tsujimoto H and Hasegawa A: "Identitication of Sporothrix schenckii based on sequences of the chitin synthase I gene"Mycoses. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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