| Project/Area Number |
12554024
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Inorganic chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
ODANI Akira Nagoya Univ., Research Center for Materials Science, associate professor, 物質科学国際研究センター, 助教授 (60143913)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKI Kiyoshi FUSO CO.LTD, Research and Development Center, manager, 研究開発センター, 研究企画室室長
荒木 清 扶桑薬品工業株式会社, 研究開発センター, 研究企画室室長
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | bioactivity / phytic acid / coordination structure / stability constant / phytase / speciation / enzyme kinetic constant / metal complex / 生理活性種 / リン酸エステル加水分解 / 抗癌活性 / イノシトールポリリン酸 / 典型金属錯体 |
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| Research Abstract |
We made the structure-property relations for metal-phytate complexes clear and showed that the specific properties of metal-phytate complexes is usefully available in biological system.
(1) Interactions of phytate with metal ions
Stability constants of various metal-phytate species were evaluated by pH titrations and were able to give the structural information as well as the spectra. These showed the importance of hydrogen bond between the phosphate group which also determines the metal coordination site.
(2) Metal ions dependence of enzyme phytase activity
Enzyme phytase from Aspergillus niger is considered to be a model of active bio-molecular receptor of phytate. This enzyme reaction rate were varied by various metal ions which assist the formation of reactive substrate of phytate at pH 6, Metal-phytate-H5. Here we demonstrated new aspect of the metal ion that the substrate of phytase is a metal complex.
(3) Bio-activity of Metal-phytate complex
Li^+ inhibition of phytase reaction and strong interaction of Li-phytate complex indicated possible relationship with the antidepressant activityof Li^+. Anti-cancer effect of various metal-phytate complexes were studied by growth inhibition of human KB cell. All metal system, especially Ca^<2+>, were more effective than phytate 12Na and this indicated Ca-phytate complex as a true active species.
These results obtained here were served a base for application of phytate and phytase to medical drugs.
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