Isolation and analysis of mutants with altered ABA recognition specificity using an ABA antagonist.
| Project/Area Number |
12640642
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
植物生理
|
|---|
| Research Institution | RIKEN (The Institute of Physical and Chemical Reseach) |
|---|
Principal Investigator |
HIRAYAMA Takashi RIEKN, Laboratory of Plant Molecular. Biology, Senior Scientist, 植物分子生物学研究室, 先任研究員 (10228819)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
|
|---|
| Keywords | Abscisic acid / mutant / ABA analogues / ABA hypersensitive mutants / HSP90 / ethanol hypersensitive mutants / archaea / シロイヌナズナ |
|---|
| Research Abstract |
In order to identify the ABA receptors, we employed a novel screening using an ABA binding inhibitor to isolate mutants that have altered binding specificity of ABA receptors. We used a ABA analogue, PBI-51, as a ABA binding inhibitor since this chemical has been reported to work as ABA binding inhibitor in Brassica napus. We tried to isolate mutants with two type of screening strategies. One is to search for mutants that seem to recognize PB151 as an ABA binding inhibitor. We isolated several candidates from 500,000 M2 seeds from EMS treated plants. However, these candidates turned out to be ABA insensitive mutants. The other strategy was to isolate mutants that seems recognize PB151 as ABA. In this screen, we isolated 8 independent lines from 400,000 M2 seeds from EMS treated plants. Those candidates show ABA-hypersensitivity, although these are not allelic to known ABA-hypersensitive mutants, eral or ein2.To increase the efficiency to isolate ABA receptor mutants, we thought out the strategy to use aba2 mutants, and isolated several dozens of candidates. ldentification of those mutated genes will surely contribute in our understanding on ABA siganling pathway. In the course to isolate ABA related mutants, we isolated a novel ethanol hypersensitiv mutant, gekol, from Arabidopsis. We identified the GEKO I gene by mapping and found it encoded a novel protein that conserved among plants and archea bacteria. GEKO I may play a pivotal role for plant sp ecific functions.
|
Report
(3 results)
Research Products
(6 results)
-
-
[Publications] Takahashi, S., Katagiri, T., Hirayama, T., Yamagchi-Shinozaki, K, Shinozaki, K.: "Hyperosmotic stress induces a rapid and transient increase in inositol 1,4,5-trisphosphate independent of abscisic acid in Arabidopsis cell culture"Plant Cell and Physiology. 42. 214-222 (2001)
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
[Publications] Takahashi, S., Katagiri, K., Hirayama, T., YamaguchiShinozaki, K., Shinozaki, K: "Hyperosmotic stress induces a rapid and transient increase in inositol 1, 4, 5-trisphospate independent of abscisic acid in Arabidopsis cell culture."Plant Cell Physiol.,. Vol.42. 214-222 (2001)
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
[Publications] Takahashi, S., Katagiri, T., Hirayama, T., Yamagushi-Shinozaki, K., Shinozaki, K.: "Hyperosmotic stress induces a rapid and transient increase in inositol 1,4,5-trisphosphate independent of abscisic acid in Arabidopsis cell culture"Plant & Cell Physiology. 42. 214-222 (2001)
