Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
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Research Abstract |
Manganese-oxidizing fungus, isolated from spring in south of Hokkaido, and manganese-oxidizing bacteria Leptothrix discophora (ATCC 43162 and 51168) were applied to removal of Mn(II) ions from solutions at neutral pHs. The manganese deposits were characterized by XRD, XRF, XPS and FE-SEM. It was found that manganese-oxidizing bacteria, L. discophora (ATCC 51168), oxidized 24 ppm of Mn(II) ions, and that the bacteria kept higher manganese-oxidizing activity in static culture than in shaking culture, because of keeping the sheath structure in the bacteria. The established medium compositions for the ATCC bacteria could not be directly applied to the oxidation of Mn(II) ions for the isolated fungus, therefore, modified. By reduction of 1/10 in concentrations of organic carbon sources in the medium, the fungus oxidized to remove over 50 ppm of Mn(II) ions from the solutions within one week. The fungus was identified to be Phoma sp. The fungus was useful to accumulate fine particles of the oxidized Mn sediments within itself. The bio-mineralized sediments were characterized to be non-crystalline Mn oxides by XRD, XRF, and XPS.
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