The biosynthesis of bioactive oxidized sorbicillin dimers
| Project/Area Number |
12660100
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
HIROTA Akira University of Shizuoka, School of Food and Nutritional Sciences, Professor, 食品栄養科学部, 教授 (40094510)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ABE Naoki University of Shizuoka, School of Food and Nutritional Sciences, Research Associate, 食品栄養科学部, 助手 (70291581)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
|
|---|
| Keywords | biosynthesis / bisorbicillinoid / Trichoderma sp. / sorbicillinol / bisorbicillinol / oxosorbicillinol / bisvertinolone / bisorbibutenolide / bisorbicillinolide |
|---|
| Research Abstract |
After Trichoderma sp. USF-2690 strain was cultured for 40 hr, the ^<13>C labeled sodium acetate was added and further additional fermentation was carried out for 24 hr. ^<13>C-NMR spectrum of sorbicillinol obtained from this fermentation showed sorbicillinol was biosynthesized via a hexaketide pathway. Next, we revealed the ^<13>Clabeled sorbicillinol was incorporated into sorbicillin, and also into bisorbicillinol by the measurrement of ^<13>C-NMR spectra of sorbicillin and bisorbicillinol.
Furthermore ^<13>C labeled oxosorbicillin was obtained from the fermentation of the addition of ^<13>C labeled sodium acetate. By addition of obtained the ^<13>C labeled oxosorbicillin into the culture broth, ^<13>C labeled bisvertinolone was yielded. The ^<13>C-NMR spectrum of ^<13>C labeled bisvertinolone proposed that bisvertinolone was biosynthesized via Michael-type reaction of sorbicillinol and oxosorbicillin, and intramolecular ketalization.
|
Report
(3 results)
Research Products
(7 results)
