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Basic Studies on Artificial Breeding of Killer Whales

Research Project

Project/Area Number 12660168
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionMie University

Principal Investigator

YOSHIOKA Motoi  Mie University, Faculty of Bioresources, Associate Professor, 生物資源学部, 助教授 (30262992)

Co-Investigator(Kenkyū-buntansha) TOBAYAMA Teruo  International Marine Biological Research Institute, Kamogawa Sea World, Director, (鴨川シーワールド), 所長(研究職)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordscetaceans / killer whale / marine mammal / artificial breeding / hormone / sperm / ovulation induction / dolphin / 海産哺乳類
Research Abstract

Initial research plan was much changed due to the difficulty of use in subject animals of killer whales in the aquarium during the study period, but we obtained following information as basic knowledge for the future success of artificial breeding of killer whales. That is ; l) we succeeded in the sperm freezing of the Pacific white-sided dolphin by applying straw method ; 2) the comparative analysis of sperm external morphology of ten cetacean species including the killer whale revealed that sperm of killer whale is long, and the head is widest and fan-shaped ; 3) sperm motion analysis by using Cell Soft software showed that post-thaw sperm of the bottlenose and Pacific white-sided dolphin have characteristics (motility, speed etc.) similar to those of sperm of humans and rabbits ; 4) ultrasonic diagnosis of gonads (testes and ovaries) for bottlenose dolphins and Pacific white-sided dolphins was attempted, and ovarian corpora, follicles and fetus were identified with the seasonal changes in testis size, and 5) hormonal study on corpora lutea and placenta suggested the source of progesterone secretion during pregnancy was both of ovarian corpus luteum and placenta in dolphins, and no pregnancy specific proteins were detected in serum samples and placental tissues from pregnant dolphins by electrophoreisis and hCG EIA, Serum levels of estrone sulfate in pregnant dolphins fluctuated with different patterns between species studied (striped dolphins and Dall's porpoise) and pregnancy stages (early and late).

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Kita, S.: "Comparative external morphology of cetacean spermatozoa"Fisheries Science. 67. 482-492 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 吉岡 基: "イルカとウミガメ"岩波書店. 178 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kita,S., Yoshioka,M., Kashiwagi,M., Ogawa,S. and Tobayama,T.: "Comparative external morphology of cetacean spermatozoa"Fisheries Science. 67(3). 482-492 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshioka,M., ed.by Y.Hayashi and K.Takeuchi: "Iruka, "Gendai Nihon Seibutsu-shi 4 -Iruka to Umigame" (in Japanese)"Iwanami-Shoten. 1-72, 178 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kita, S.: "Comparative External Morphology of Cetacean Spernatozoa"Fisheries Science. 67. 482-492 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 吉田 基: "イルカとウミガメ"岩波書店. 178 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kita,S.: "Comparative External Morphology of Cetacean Spermatozoa "Fisheries Science. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 吉岡基: "イルカとウミガメ"岩波書店. 178 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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