| Project/Area Number |
12660257
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
YOSHIMURA Yukinori Faculty of Applied Biological Science, Hiroshima University, Professor, 生物生産学部, 教授 (10167017)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | bird / salmonella / reproductive immunology / estrogen / MHC mRNA / IgG mRNA / egg / oviduct / IgG mRNG / ニワトリ / サルモネラ / 免疫 / 卵巣 / 生殖器 / 内分泌 |
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| Research Abstract |
One of the recent problems in poultry industry is the contamination of birds and eggs by salmonella organisms. Generally, the process of immune response to foreign agents includes, antigen presentation by major histocompatibility complex (MHC) class II expressing cells, stimulation of T cells by this MHC signals followed by B cell activation, and antibody production by B cells. It is important to enhance immune functions in the reproductive organs to protect the infections and contamination of birds themselves and eggs. The aim of this study was to determine the mechanism by which immune functions in the reproductive organs are regulated by gonadal steroids and how the immune function play roles in protection of reproductive organs from salmonella antigen. The results showed that the mRNAs encoding IgG and MHC class II were expressed in the oviduct. Estrogen increased IgG mRNA expression as well as the population of T and B cells, and also it increased yolk IgY. Such enhancive effect of estrogen on immune functions occurred specifically in the target organs for estrogen. Furthermore, responsiveness of immune functions to salmonella antigen was confirmed because the injection of this antigen enhanced MHC class II expression in the ovary. These results suggested that the oviduct is the inducible site of immunoreactions that are enhanced by estrogen, and the immune response to salmonella antigen could be also induced in these organs.
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