Effects of radiation on expression of NT-3 gene or right-left asymmetrical development related genes
| Project/Area Number |
12670034
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Hiroshima International University |
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Principal Investigator |
SUMIDA Hiroshi Hiroshima International University, Health Sciences, Professor, 保健医療学部, 教授 (10154626)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | radiation / X-ray / neutron / Californium / development / homeobox genes / NT-3 / Pitx2 / PitX2 / Ets-2 / 大血管転換 / 中枢神経系異常 / カルフォルニウム / 四肢異常 |
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| Research Abstract |
The effects of high energy X-ray to White Leghorn embryos, or ^<252>Cf or 3 MeV neutron to mouse embryos on morphogenesis were studied. In order to check effects of radiation on neural crest cells, expression of a NT-3 gene was checked. Also, expression of a Pitx2 gene regarding to the right-left asymmetrical development was checked.
As the results, the right and left axis of a embryo was reversed by neutron irradiation. Decreasing of NT-3 gene expression by high energy X-ray was recognized. The degree of decreasing of gene expression was different in the embryos. Expression of the Pitx2 gene was also decreased after irradiation. Also, the cases that suggests mutation of the Pitx2 gene was recognized. It was indicated that expression of certain genes such as NT-3 were especially affected by radiation. Certain homeobox genes, however, were not mutated by radiation.
Besides, expression of genes regarding to the cell defense to radiation was studied. I examined effects of high energy X-ray to chicken embryos on expression of p53, ATM and RAD51. After 24 hr of 5 Gy or 8 Gy irradiation, expression of p53 and ATM gene was decreased significantly compared with controls. P53 and ATM are the important gene regarding to G_1 arrest and cell death by radiation irradiation, thus the results suggested that radiation affected cell defense system. Expression of RAD51 genes was increased to 2 times with controls after radiation irradiation. This result is different from a previous reports, thus, it is suggested that radiation irradiation activates DNA recombinant repair system.
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Report
(4 results)
Research Products
(6 results)
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[Publications] Kumasaki, T., Sasaki, M., Nishiyama, M., Teranishi, Y., Sumida, H. and Mitsui, Y.: "Effect of Bcl-2 down-regulation on cellular life span"Biogerontology. 3. 291-300 (2002)
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