| Project/Area Number |
12670074
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
SATO Yuko Tokyo Metropolitan Institute of Gerontology, Assistnat researchoer of Dept. Clini. Physiol., 臨床生理, 助手 (70158939)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Minoru Tokyo Metropolitan Institute of Gerontology, Assistnat researchoer of Dept. Clini. Physiol., 臨床生理部門, 助手 (70133634)
KANAI Setsuko Tokyo Metropolitan Institute of Gerontology, Assistnat researchoer of Dept. Clini. Physiol., 臨床生理部門, 助手 (90100122)
MIYASAKA Kyoko Tokyo Metropolitan Institute of Gerontology, Head of Dept. Clini.Physiol., 臨床生理部門, 研究室長 (90166140)
MASUDA Masao Tokyo Metropolitan Institute of Gerontology, Assistnat researchoer of Dept. Clini. Physiol., 臨床生理部門, 助手 (20260284)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | triglyceride / CCK-A receptor / Apolipoprotein-A / NPY / Intestinal transport / diacylglycerol / energy expenditure / apolipoproteinA-IV |
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| Research Abstract |
OLETF rats are a model for NIDDM, which is first manifested at 18 weeks of age. We assessed age-related changes in lymphatic lipid transport in the intestine of OLETF rats and compared them with those of control LETO rats. Olive oil was infused into thterats with a mesenteric lymph fistula, which was created under ethrane anesthesia. A significant increase in lymphatic triglyceride transport in OLETF rats was observed at 18-19 weeks of age, compared with under 17 weeks of age, but no age-related change was observed in LETO rats. Food restriction, exercise training, or troglitazone treatment in OLETF rats prevented the age related increase in lipid transport. Biliary phosphatidylcholine concentration was higher in OLETF rats than in LETO rats, but no difference was seen in bile acid concentrations of the activity of microsomal TG transfer protein between the two strains. This study shows that increased lipid transport in the intestine may occur in NIDDM.
The diurnal profile of energy expenditure in OLETF rats was different from LETO rats after NIDDM manifested. Treatments with food restriction, exercise, or troglitazone normalized diurnal profiles.
Oral administration of diacylglycerold increased CCK release in humans, but the response was not different from that induced by triglyceride.
Gallstone formation was observed when they were raised with high fat and high protein diet in CCK-A receptor knockout mice.
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