| Project/Area Number |
12670347
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Yamanashi Medical University |
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Principal Investigator |
WANG Peiyu Yamanashi Medical University, Department of Envirorment Health, Research Associate, 医学部, 助手 (10283201)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Takashi Yaroanashi Medical University, Department of Environrent Health, Associate Professor, 医学部, 助教授 (10233876)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Diabetes / Insulin / glucose / glucose tolerance / インスリン / 血糖 / 耐糖能 / IPGTT |
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| Research Abstract |
Aims/hypothesis, The aim of the study was to determine the effect of high-fat/low-carbohydrate (HF/LC) diet on the onset of type 2 diabetes mellitus in normal rats.
Methods. Male Wistar Imamichi rats were kept on a Control (carbohydrate 60 %, fat 15 %) or HF/LC (carbohydrate 10%, fat 65 %) diet for 17 months. Intraperitoneal glucose tolerance test was performed once every 2 months. At the end of the experiment, rats were decapitated and plasma triglyceride, non-esterified fatty acid, high density lipoprotein-Cholesterol, total and free cholesterol levels were measured.
Results. Glucose tolerance was impaired after 2 months of feeding in the HF/LC rats, accompanied by decrease of the postload plasma insulin secretion and. Along with time till 16-month of HF/LC diet feeding, the glucose tolerance was further deteriorated with more serious impairment of insulin secretion, 16 of 18 rats became diabetic in HF/LC group whereas only 4 of 17 rats were diabetic in Control group.
Conclusions/interpretation. Our results demonstrate that long-term HF/LC diet feeding decrease insulin secretion and sensitivity, induces diabetes mellitus in HF/LC diet feeding rats.
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