| Project/Area Number |
12670401
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, DEPARTMENT OF LEGAL MEDICINE |
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Principal Investigator |
YOSHIMOTO Kanji Kyoto Prefectural University of Medicine, Department of Legal Medicine, Assistant Professor, 医学部, 講師 (70111903)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | ISCHEMIA / DOPAMINE / SEROTONIN / ANIMAL MODEL |
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| Research Abstract |
In the present study, we used in vivo brain micro dialysis to examine the effects of stimulations of ischemia and/or potassium on the release of dopamine (DA) and serotonin (5-HT) in the nucleus accumbens (ACC) of anesthetized rats. Ischemia (Four vessels occlusion : 4VO) for 10 min increased DA and 5-HT release in the ACC 200-fold and 15-fold, respectively , in the first experiment. In second experiment, releases of DA and 5-HT in the ACC increased 350-400 % and 150-180 % by first and second K^+ stimulation, respectively, in the K^+-K^+ group. The 4VO treatment induced significant and massive increases of DA and 5-HT releases in the ACC, following K+ stimulation increased 5-HT release in the Ischemia-K^+ group compared with the K^+-K^+ group. Brain vulnerability was shown in the dopaminergic and serotonergic neurons in the same area of the ACC. These findings suggested that the ACC neurons showed a resistance to dysfunction in 10 min-ischemia and maintained neural function, monoamine synthesis and neurotransmitter releases within the transient neural damage.
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