| Project/Area Number |
12670513
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kitasato University |
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Principal Investigator |
GOSO Yukinobu Kitasato Univ. Sch. Med., Dept. Biochem., Assistant Professor, 医学部, 講師 (20112659)
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| Project Period (FY) |
2000 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Helicobacter pylori / Monoclonal antibody / Mucus gel / Gastric mucus / Gastric mucosa / Oligosaccharides / Mucin / 粘膜ゲル層 / ネオ糖脂質 / MALDI-TOF-MS |
|---|
| Research Abstract |
In order to examine the role of the gastric mucus on Helicobacter pylori (Hp) infection, the following studies have been performed.
1. Binding of human gastric mucins with Hp was assayed with the surface plasmon biosensors. The results indicated that Hp bound strongly to the gastric mucins obtained from the corpus compared to those from the antrum.
2. A novel anti-mucin monoclonal antibody (mAb), designated RGM23, was developed and characterized. The histochemical and biochemical studies showed that RGM23 recognized the peptide moiety of MUC5AC mucin present in the surface mucosa of the stomach. Combined use of RGM23 and HIK1083, which recognize the mucin present in the glandular mucosa, may permit to get the surface and glandular mucins separately. This may enable to reveal a role of each mucin for Hp infection.
3. To determine the mucin oligosaccharides recognized by Hp, binding assay methods were developed. Intact oligosaccharides were obtained from the mucins by gas-phase hydrazynolysis and then converted to neoglycolipid. After the neoglycolipids were separated by thin-layer chromatography, their mass were determined by MALDI-TOF-MS. Hp bind to oligosaccharides on TLC plates, thus enabling to determine structure of the oligosaccharides recognized by Hp.
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