| Project/Area Number |
12670531
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka Medical College |
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Principal Investigator |
SAITOH Osamu Osaka Medical College, Faculty of Medicine, Lecturer, 医学部, 講師 (40186929)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Keishi Osaka Medical College, Faculty of Medicine, fellow, 医学部, 専攻医
KAYAZAWA Masanobu Osaka Medical College, Faculty of Medicine, staff, 医学部, 助手 (10340548)
KEN Nakagawa Osaka Medical College, Faculty of Medicine, staff, 医学部, 助手 (00278532)
HOSHIMOTO Masahiro Osaka Medical College, Faculty of Medicine, fellow, 医学部, 専攻医
TANAKA Seigou Osaka Medical College, Faculty of Medicine, fellow, 医学部, 専攻医
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | intestinal mucosa / intestinal epithelial cell / whole gut lavage fluid / barrier / permeability / fatty acid / Caco-2 cells / IL-8 / K^+-channel / Caco-2細胞 / T84細胞 |
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| Research Abstract |
1. Effect of fatty acids on chemokine production in intestinal epithelial cells, Caco-2 cells
Butyrate, a short-chain fatty acid, decreased interleukin(IL)-8 production in intestinal epithelial cells, ω-3 fatty acids such as docosahexaenoic acid (DMA) and eicosapentaenoic acid (ERA) showed no effect on IL-8 production. Oleic acid (OA) and capric acid (CA) increased IL-8 production. Protein kinase C and phospholipase A2 were involved in signal transduction pathways for OA-induced IL-8 production but not for CA-induced IL-8 production.
2. Measurement of proteins in whole gut lavage fluid (WGLF) and feces
Either WGLF or feces can be used as a sample to investigate pathophysiology and disease activity in patients with inflammatory bowel disease (IBD). In active IBD, the concentrations of granular proteins (lactoferrin, myeloperoxidase, PMN-elastase, lysozyme) were increased both in the supernatant and pellet. These findings suggested that although the migration of neutrophils into the lumen is extremely increased in active IBD, a number of neutrophils that migrated into the lumen did not release granular proteins. And lactoferrin is the most suitable of these proteins for use as a neutrophilderived WGLF marker of intestinal inflammation.
3. Effects and cellular actions of oxidants on anion secretion in human colonic T84 cells.
Oxidants potentiate both Ca^<2+>- and CAMP-stimulated Cl^- secretion by a direct effect on calcium-activated basolateral K^+ channel conductance, lowering its Ca^<2+> activation threshold. This effect may play an important role in amplifying and prolonging the secretory response of inflamed intestinal mucosa and enhancing the severity of diarrhea.
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