| Project/Area Number |
12670685
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kagawa Medical University |
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Principal Investigator |
KOHNO masakazu Kagawa Medical University, The Second Department of Internal Medicine, Professor, 医学部, 教授 (20153489)
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| Co-Investigator(Kenkyū-buntansha) |
YUKIIRI kazushi Kagawa Medical University, The Second Department of Internal Medicine, Assistant, 医学部附属病院, 助手 (30346647)
TAKAGI yuichiro Kagawa Medical University, The Second Department of Internal Medicine, Assistant, 医学部附属病院, 助手 (20346646)
OHMORI koji Kagawa Medical University, The Second Department of Internal Medicine, Assistant Professor, 医学部附属病院, 講師 (00263913)
和田 佳宏 香川医科大学, 医学部・附属病院, 助手 (00314930)
藤田 憲弘 香川医科大学, 医学部・附属病院, 助手 (40322268)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | endothelin / endothelin converting enzyme / statin / EPA / HGF / basic FGF / angiotensin II / atherosclerosis / 動脈硬化症 / 遺伝子治療 / 高血圧 / 臓器障害 |
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| Research Abstract |
A vasoconstrictive and growth-promoting peptide, endothelin-1, is found to play a role in the initiation and development of atherogenesis. The objectives of the present study were to determine whether statin, eicosapentaenoic acid (EPA) can affect endothelin-1 production and also determine whether hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF) can affect endothelin-1 production. Media concentrations of endothelin-1 were measured by a radioimmunoassay using the polyclonal antibody for synthetic endothelin-1. Fetal calf serum (FCS) and angiotensin II stimulated endothelin-1 production in a concentration-dependent manner in these cells. Statin and EPA inhibited endothelin-1 production. On the other hand, HGF did not affect endothlin-1 production significantly. HGF also did not affect endothelin-1 production. These results suggest that statin and EPA exert antiatherosclerotic effect through inhibiting endothelin-1 production and that HGF, but not basic FGF, did not affect endothelin-1 production of cultured rat vascular SMCs, and the HGF may be more advantageous for clinical application than basic FGF.
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