| Project/Area Number |
12670814
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Shinshu University |
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Principal Investigator |
TANIGUCHI Shun'ichiro Shinshu University, School of Medicine, Professor, 医学部, 教授 (60117166)
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| Co-Investigator(Kenkyū-buntansha) |
SAIDA Toshiaki Shinshu University, School of Medicine, professor, 医学部, 教授 (10010381)
SAGARA Junji Shinshu University, School of Medicine, associate professor, 医学部, 助教授 (10225831)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Hyaluronate / Has1,2 / human melanoma / nude mice / transplantation of tumor / tumor formation / ASC / moesin / 合成酵素遺伝子HAS1、HAS2 / HAS1あるいはHAS2発現 / dormant state / アポトーシス / 遺伝子ASC / HAS / 遺伝子導入 / 細胞運動性 / 造腫瘍性 |
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| Research Abstract |
1. By transplanting human melanoma cell clones transferred with expression vector carrying Has1,2 which codes hyaluronate synthetase, we obtained following results concerning in vivo phenotypes of the cells.
(1) In addition to our previous report that Has1,2 transfected W793, a human melanoma cell line, was enhanced in cell motility(J.I.D , 1995), we confirmed the phenomenon by in dependent and different type of Has1,2 expression vector.
(2) When we i.p. transplanted the newly established transfectants into immunodeficient nude mice (Balb/c), they formed tumor nodules 16-18 weeks after the transplantation with ascites. As for the mock transfectants, that was not the case even one year after the transplantation. By s.c. transplantation of the cells, we confirmed the tumor formation only in Has1, 2 transfectants.
2. Immunohistochemical staining of human melanoma tissues has been unsuccessful due to the lack of sufficiently specific antibodies of Has1, 2.
3. According to the results show in (1), resistance to apoptosis was regarded as one of the main of phenotypes acquired in the transfectants. When we investigated ASC gene (found by ourselves in 1999, JBC), it was very frequently and specifically down-regulated in human melanoma tissues.
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