Association of GRO-α in the genesis of malignant melanoma

• Project/Area Number: 12670841
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: KAWASHIMA Makoto Dept.of Dermatology,Tokyo Women's Medical University,Professor, 医学部, 教授 (60114451)
• Co-Investigator(Kenkyū-buntansha): SHISHIDO Etsuko Dept.of Dermatology,Tokyo Women's Medical University,Assistant, 医学部, 助手 (60307543)
• Project Period (FY): 2000 – 2001
• Project Status: Completed (Fiscal Year 2001)
• Budget Amount: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: malignant melanoma / growth related oncogene α / endothelin / stem cell factor / hepatocyte growth factor / RT-PCR / Growth regulated oncogene α / GROα / 腫瘍

Research Abstract

Malignant melanoma is a poor prognostic skin tumor, which recurs and metastasizes frequently. Thus, it is important to know the mechanism of its genesis and progression. We studied the association of several factors which might have some roles in melanogenesis and genesis of that tumor, in comparison with other pigmentary skin disorder and tumors.
In case of senile lentigo, keratinocyte derived Endothelin 1 is shown to be associated to melanogenesis. The pigmentation of overlying epidermis of dermatofibroma is brought by activation of melanocyte through up-regulation of Stem cell factor and Hepatocyte growth factor. In basal cell carcinoma, Stem cell factor was suspected to be associated with its hyperpigmentation. The role of GRO-α in the genesis of malignant melanoma was studied in several cell lines, and we found the expression of mRNA in most cell lines by RT-PCR. This findings may suggest the important role of GRO-α in transformation and progression of melanoma cells.

Research Products

• [Publications] Satsuki Kadono, Izumi Manaka, Makoto Kawashima, Takashi Kobayashi, Genji Imokawa: "The role of the epidermal endothelin cascade in the hyper pigmentation mechanism of lentigo senilis"J Invest Dermatol. 116. 571-577 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Etsuko Shishido, Satsuki Kadono, Izumi Manaka, Makoto Kawashima, Genji Imokawa: "The mechanism of epidermal hyperpigmentation in dematofibroma is associated with stem cell factor and hepatpcyte growth factor expression"J invest Dermatol. 117. 627-633 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Satsuki Kadono, Izumi Manaka, Makoto Kawashima, Takashi Kobayashi, | and Genji Imokawa: "The role of the epidermal endothelin cascade> in the hyperpigmentation mechanism of lentigo senilis"J. Invest. T. l-WT (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Etsuko Shishido, Satsuki Kadono, Izumi Manaka, Makoto Kawashima, and Genji Imokawa: "The mechanism of epidermal hyperpigmeritation in dermatofibroma is associated with stem cell factor and hepatpcyte growth factor expression"T invest. 7. 27-633 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] S.Kadono, I.Manaka, M.Kawashima, T.Kobayashi, G.Imokawa: "The role of epidermal endothelin cascade in the hyperpigmentation mechanism of lentigo senilis"J.Invest. Dermatol.. 116・4. 571-577 (2001)
• [Publications] E.Shishido, S.Kadono, I.Manaka, M.Kawashima, G.Imokawa: "The mechnism of epidermal hyperpigmentation in dermatofibroma is associated with stem cell factor and hepatocyte growth factor expression"J.Invest.Dermatol.. 117・3. 627-633 (2001)