| Project/Area Number |
12671071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | NATIONAL INSTITUTE OF Neuroscience, NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY(NCNP) |
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Principal Investigator |
SAKURAGAWA Norio NATIONAL INSTITUTE OF Neuroscience, NCNP, DEPARTMENT OF METABOLIC DISEASES DIRECTOR, 神経研究所・疾病研究第五部, 部長 (70183374)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | FERAL DISTRESS / AMNIOTIC FLUID / CATECHOLAMINES / NEUROTROPHIC FACTORS / ACTIVIN / 神経伝達物資 / アクチビン / animal cap assay |
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| Research Abstract |
TO INVESTIGATE THE BIOCHEMICAL MARKERS OF FETAL DISTRESS, WE EXAMINED NEUROTROPHIC FACRTORS IN AMNIOTIC FLUID FROM RATS RECEIVED TRANSIENT UTERIN ISCHEMIA. WE USED PREGNANCY 18 WISTER RATS, WHICH RIGHT UTERINE ARTERIS CRAMPED 30 MINUTES. AMNIOTIC FLUID OBTAINED BY AMNIOCENTESIS AFTER 24 HRS FROM CRAMPED. FOR EACH EXPERIMENT THE AMNIOTIC FLUID IN THE RIGHT HORN SERVED AS A ISCHEMIA GROUP AND THOSE IN THE LEFT HORN WERE USED AS A CONTROL GROUP. MEAHWHILE, WE PERFORMED IN VITRO EXPERIMENT FOR HYPOXIA. WE USED HUMAN AMNIOTC EPITHELAL CELLS DERIVED FROM PLACENTA BY CAESAREAN SECTION. CONTROL GROUP CULTURED IN 5% C02 INCUBATOR AT 37^<0>C FOR 48 HRS, ISCHEMIA GROUP INCUBATED IN 2% O_<2>, 5% CO_<2> INCUBATOR 37^<0> : FOR 48 HRS. STATISTICAL ANAYSIS BY WILCOXON SIGNED-RANK TEST DEMONSTRATED A SIGNIFICANT INCREASE IN NT-3 CONCENTRATIONS IN AMNIOTIC FLUID FROM THE ISHCEMIC GROUP IN CONTRAST NGF AND BDNF CONCENTRATIONS WHICH ALSO INCREASED BUT NOT SIGNIFICANT. IN VITRO EXPERIMNT SHOWED THAT NT-3 IN CULTURED MEDIUM OF HYPOXIC CELLS INCREASED SIGNIFICATLY. THESE RESULTS INDICATE THAT NT-3 IN AMNIOTIC FLUID MAY BE USED AS ONE OF BIOCHEMICAL MARKERS FOR FETAL DISTERSS.
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