| Project/Area Number |
12671089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Nagasaki University |
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Principal Investigator |
ASHIZAWA Kiyoto School of Medicine, Nagasaki University, Assistant, 医学部, 助手 (10304932)
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| Co-Investigator(Kenkyū-buntansha) |
SERA Nobuko Nagasaki University, Hospital, Senior resident, 医学部・附属病院, 医員
EJIMA Eri Nagasaki University, Hospital, Assistant, 医学部・附属病院, 助手 (30231187)
KATSUMI Eguchi School of Medicine, Nagasaki University Professor, 医学部, 教授 (30128160)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | thyroid cancer / metastasis / cyclooxygenase-2 (COX-2) / invasiveness / Laminin / KAI-1 / bone metastasis / animal model / 転移機構 / 遊走能 / 細胞接着因子 / オステオポンチン / 乳頭癌株 / 癌転移 / Cyclooxygenase-2(COX-2) / COX-2選択的阻害剤 / 細胞死 / 乳頭癌 / 濾胞癌 / KAI1 |
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| Research Abstract |
Metastasis is the chief cause of morbidity and mortality in most cancers. We addressed the mechanism of metastasis of thyroid cancer. As there are many factors, which modulate the metastasis, we mainly assessed three points as follows in this study:
1. Expression of cyclooxygenase-2 (COX-2) in thyroid cancers and the effect of COX-2 on proliferation of thyroid cancer cell
2. In vitro invasiveness of thyroid cancer cell
3. Expression of tumor suppressor gene in thyroid cancers
[Result]
1. Cox-2 was highly expressed in thyroid cancers and moderately in thyroid adenoma, but barely expressed in normal and Graves' thyroid gland. Selective COX-2 inhibitor, NS-398 suppressed the growth of COX-2 expressing cells, such as NPA cells (human papillary thyroid carcinoma cell line), and WRO cells (human follicular thyroid cancer cell line). These results indicated that COX-2 is involved in the progression of thyroid cancer and COX-2 inhibitor might be useful for thyroid cancer therapy.
2. Laminin, major component of the basement membrane of epithelia and endothelia, strongly stimulated tumor invasiveness in TPC-1 cells (human papillary thyroid carcinoma cell line) by chemotaxis chambers. Laminin also stimulated invasiveness in WRO cells (human follicular thyroid cancer cell line), but not FRO cells (human anaplastic thyroid cancer cell line). These results indicated that Laminin is involved in the invasiveness of thyroid cancer.
3. KAI-1 (CD82) is one of the genes, which relate with metastasis, especially lymphnode metastasis. As papillary carcinoma has tendency to invade lymphnode, we compared the expression of KAI-1 (CD82) between in the papillary carcinomas and normal thyroid tissue. However we found no difference of expression between normal and malignant lesions.
To study the mechanism of the metastasis of thyroid cancer, we will make animal models of metastatic thyroid cancer.
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