Tolerance induction using unique immune response in the liver
| Project/Area Number |
12671226
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
DONO Keizo Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (60283769)
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Isao Osaka University Hospital, Medical Staff, 医学部・附属病院, 医員
NAGANO Hiroaki Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (10294050)
SAKON Masato Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (40170659)
清水 潤三 大阪大学, 医学部・附属病院, 医員
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Allo antigen inoculation / Rat liver transplantation / Immune response in the liver / microchimerism / microchimerism / maicrochimerism |
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| Research Abstract |
The liver has been believed to be an unique organ form the immunological point of view, Because it is well known that the liver could be accepted more easily than other organ grafts. The liver is believed to have tolerogeneic effect. In this study, we investigated the immune response in the liver using syngeneic liver transplantation.
Rejection to cardiac allografts were accelerated when the liver was transplanted from syngeneic rats which were given donor splenocytes intravenously 1day before. On the other hand, the rats which were hepatectomised one day after injection of splenocytes and transplanted naive syngeneic liver grafts had tendency to accept allo cardiac grafts.
When hepatectomy was done two days after antigen injection, this phenomenon was disappeared. Irradiation of the spleen cells before inoculation did not alter the effect. But depletion of phagocytic activity of Kupffer cell eliminate the unique immune response in the liver.
This results suggests that the Kupffer cells play an key role in the immune pathway in the liver. Immunological tolerance to the liver allografts could be induced using this phenomenon.
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Report
(3 results)
Research Products
(14 results)
