| Project/Area Number |
12671246
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | YOKOHAMA CITY UNIVERSITY, SCHOOL OF MECICINE |
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Principal Investigator |
SEKIDO Hitoshi YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF SURGERY II, ASISTANT PROFESSOR, 医学部, 講師 (90187849)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHIZAKI Yoshide RIKEN CENOMIC SCIENCES CENTER, GENOMIC EXPLORATION RESEARCH GROUP, PROJECT DIRECTOR, ゲノム科学総合研究センター, プロジェクトディレクター (70192705)
ICHIKAWA Takashi YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF SURGERY II, ASISTANT PROFESSOR, 医学部, 助手 (80275049)
ICHIKAWA Yasushi YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF SURGERY II, ASISTANT PROFESSOR, 医学部, 助手 (70254208)
OKAZAKI asushi RIKEN GENOMIC SCIENCES CENTER, GENOMIC EXPLORATION RESEARCH GROUP, TEAM LEADER, ゲノム科学総合研究センター, チームリーダー (80280733)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Pancreaticobiliary maljunction / Micro array / cDNA / Carcinogenesis |
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| Research Abstract |
The mechanism of gastrointestinal carcinogenesis was comprehensively analyzed using cDNA micro array. Materials were the cancerous or non-cancerous mucosa at the gallbladders or common bile ducts taken from surgical specimen of the patients with pancreaticobiliary maljunction (PBMJ). RNAs were extracted from these samples. Because the quantity of the RNA was considerably small, amplification was performed according to the linear amplification method using Amino MEGA Script T7 kit. Applying this technique, the enough RNAs were obtained form 1.5 to 3 micrograms of total RNA. The linearity analysis between before and after the amplification was performed and well correlation was confirmed, correlation coefficient 0.7. Comprehensive analyses of gene expressions were performed using RNA samples extracted from the surgical specimens in the patients with PBMJ. The changes of gene expression patterns were comprehensively detected by cDNA micro array. Comparing these changes between colonic adenomas and the biliary mucosae in PBMJ, this study may contribute to elucidating the mechanism of gastrointestinal carcinogenesis.
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