Study for the mechanism of the development of co-lateral vein in portal hypertension

Research Project

Project/Area Number	12671279
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Digestive surgery
Research Institution	HYOGO COLLEGE OF MEDICINE
Principal Investigator	TAKEUCHI Masaharu (2001) Hyogo College of Medicine, Reserch Associate, 医学部, 助手 (00258162) 中井謙之 (2000) 兵庫医科大学, 医学部, 講師 (50198024)
Co-Investigator(Kenkyū-buntansha)	UEKI Takahiro Hyogo College of Medicine, Research Associate, 医学部, 助手 (10309461) FUJIMOTO Jiro Hyogo College of Medicine, Professor, 医学部, 教授 (90199373) 竹内雅春兵庫医科大学, 医学部, 助手 (00258162)
Project Period (FY)	2000 – 2001
Project Status	Completed (Fiscal Year 2001)
Budget Amount *help	¥3,400,000 (Direct Cost: ¥3,400,000) Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000) Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywords	portal hypertension / bone marrow transplantation / Lac-Z transgenic mouse / 新生血管 / Lac-Z transgeuic mouse / Lac-Z transgenic mouse / 肝硬変
Research Abstract	Five-week-old syngeneic BL/6 mouse served as donors for Lac-Z transgenic mouse. Bone marrow transplantation was performed.After whole body irradiation with 900cOy. Fibrotic liverwas induced by administration of OCL4. Hepatocyle growth factor(HGF) gene transfer was performed and examined if bone marrow cell would be delivered to liver or not in regeneration of liver. We got the following finds. Hepatocytes or oval cells did not derive from hematopoietic stem cell in hepatic regeneration in a little damaged liver. After hematopoietic stem cell was delivered to the endothelial cell, the liver was reconstructed. In this experiment, the co-lateral vein developed but hematopoietic cell was not found. We could not show the mechanism of the development of co-lateral vein in portal hypertension.